Anti-ABCA2 antibody (Internal) (STJ71599)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-ABCA2 (Internal) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000.WB: Preliminary experiments gave an approx 35kDa band in Human Brain (Amygdala, Frontal Cortex, Hippocampus and Substantia Nigra) lysates after 0.3µg/ml antibody staining. Please note that |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ABCA2 |
| Gene ID | 20 |
| Uniprot ID | ABCA2_HUMAN |
| Accession Number | NP_001597.2; NP_997698.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | KKQSDNLEQQETEP |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001597.2; NP_997698.1). |
Additional Info
| Post Translational Modifications | Methylated at Gln-271 by N6AMT1. |
| Function | Probable lipid transporter that modulates cholesterol sequestration in the late endosome/lysosome by regulating the intracellular sphingolipid metabolism, in turn participates in cholesterol homeostasis (Probable). May alter the transbilayer distribution of ceramide in the intraluminal membrane lipid bilayer, favoring its retention in the outer leaflet that results in increased acid ceramidase activity in the late endosome/lysosome, facilitating ceramide deacylation to sphingosine leading to the sequestration of free cholesterol in lysosomes. In addition regulates amyloid-beta production either by activating a signaling pathway that regulates amyloid precursor protein transcription through the modulation of sphingolipid metabolism or through its role in gamma-secretase processing of APP. May play a role in myelin formation. |
| Protein Name | Atp-Binding Cassette Sub-Family A Member 2Atp-Binding Cassette Transporter 2Atp-Binding Cassette 2 |
| Database Links | Reactome: R-HSA-1369062 |
| Cellular Localisation | Endosome MembraneMulti-Pass Membrane ProteinLysosome MembraneForms DiscretePunctate Intracellular Vesicles |
| Alternative Antibody Names | Anti-Atp-Binding Cassette Sub-Family A Member 2 antibodyAnti-Atp-Binding Cassette Transporter 2 antibodyAnti-Atp-Binding Cassette 2 antibodyAnti-ABCA2 antibodyAnti-ABC2 antibodyAnti-KIAA1062 antibody |
Information sourced from Uniprot.org