• Immunofluorescence analysis of paraffin-embedded mouse brain using ABCA2 Rabbit polyclonal antibody (STJ119146) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-251 MG cells using ABCA2 Rabbit polyclonal antibody (STJ119146) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ABCA2 Rabbit polyclonal antibody (STJ119146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ABCA2 antibody (140-350) (STJ119146)

SKU:
STJ119146

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCA2 (140-350) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCA2
Gene ID: 20
Uniprot ID: ABCA2_HUMAN
Immunogen Region: 140-350
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-350 of human ABCA2 (NP_001597.2).
Immunogen Sequence: SHLDRSTVSSFSLDSVARNP QELWRFLTQNLSLPNSTAQA LLAARVDPPEVYHLLFGPSS ALDSQSGLHKGQEPWSRLGG NPLFRMEELLLAPALLEQLT CTPGSGELGRILTVPESQKG ALQGYRDAVCSGQAAARARR FSGLSAELRNQLDVAKVSQQ LGLDAPNGSDSSPQAPPPRR LQALLGDLLDAQKVLQDVDV LSALALLLPQG
Tissue Specificity Isoform 3: Highly expressed in the brain,peripheral blood leukocytes and ovary, whereas lower levels of expression is observed in kidney and liver. Isoform 4: Weakly expressed in brain and highly in peripheral blood leukocytes.
Post Translational Modifications Methylated at Gln-271 by N6AMT1.
Function Probable lipid transporter that modulates cholesterol sequestration in the late endosome/lysosome by regulating the intracellular sphingolipid metabolism, in turn participates in cholesterol homeostasis (Probable). May alter the transbilayer distribution of ceramide in the intraluminal membrane lipid bilayer, favoring its retention in the outer leaflet that results in increased acid ceramidase activity in the late endosome/lysosome, facilitating ceramide deacylation to sphingosine leading to the sequestration of free cholesterol in lysosomes. In addition regulates amyloid-beta production either by activating a signaling pathway that regulates amyloid precursor protein transcription through the modulation of sphingolipid metabolism or through its role in gamma-secretase processing of APP. May play a role in myelin formation.
Protein Name Atp-Binding Cassette Sub-Family A Member 2
Atp-Binding Cassette Transporter 2
Atp-Binding Cassette 2
Database Links Reactome: R-HSA-1369062
Cellular Localisation Endosome Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Forms Discrete
Punctate Intracellular Vesicles
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family A Member 2 antibody
Anti-Atp-Binding Cassette Transporter 2 antibody
Anti-Atp-Binding Cassette 2 antibody
Anti-ABCA2 antibody
Anti-ABC2 antibody
Anti-KIAA1062 antibody

Information sourced from Uniprot.org

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