Western blot analysis of various lysates using GABA transaminase (GABA transaminase (ABAT) ) Rabbit mAb (STJ11102437) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ABAT antibody (1-100) [S7MR] (STJ11102437)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102437
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General Information

Short DescriptionRabbit monoclonal ABAT (1-100) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS7MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolABAT
Gene ID18
Uniprot IDGABT_HUMAN
Immunogen Region1-100
Immunogen SequenceMASMLLAQRLACSFQHSYRL LVPGSRHISQAAAKVDVEFD YDGPLMKTEVPGPRSQELMK QLNIIQNAEAVHFFCNYEES RGNYLVDVDGNRMLDLYSQI
SpecificityA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABA transaminase (ABAT) (P80404).

Additional Info

Tissue Specificity Liver > pancreas > brain > kidney > heart > placenta.
Function Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
Protein Name 4-Aminobutyrate Aminotransferase - Mitochondrial
(S-3-Amino-2-Methylpropionate Transaminase
Gaba Aminotransferase
Gaba-At
Gamma-Amino-N-Butyrate Transaminase
Gaba Transaminase
Gaba-T
L-Aibat
Database Links Reactome: R-HSA-916853
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-4-Aminobutyrate Aminotransferase - Mitochondrial antibody
Anti-(S-3-Amino-2-Methylpropionate Transaminase antibody
Anti-Gaba Aminotransferase antibody
Anti-Gaba-At antibody
Anti-Gamma-Amino-N-Butyrate Transaminase antibody
Anti-Gaba Transaminase antibody
Anti-Gaba-T antibody
Anti-L-Aibat antibody
Anti-ABAT antibody
Anti-GABAT antibody

Information sourced from Uniprot.org

Citations

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