• Immunohistochemistry analysis of AASS in paraffin-embedded rat liver using AASS Rabbit polyclonal antibody (STJ11105411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AASS in paraffin-embedded mouse kidney using AASS Rabbit polyclonal antibody (STJ11105411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AASS in paraffin-embedded human liver using AASS Rabbit polyclonal antibody (STJ11105411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Rat kidney, using AASS Rabbit polyclonal antibody (STJ11105411) at 1:100 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-AASS antibody (480-600) (STJ11105411)

SKU:
STJ11105411

Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Alpha-Aminoadipic Semialdehyde Synthase-Mitochondrial (480-600) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:50-1:200
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AASS
Gene ID: 10157
Uniprot ID: AASS_HUMAN
Immunogen Region: 480-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 480-600 of human AASS (NP_005754.2).
Immunogen Sequence: RRKVLVLGSGYISEPVLEYL SRDGNIEITVGSDMKNQIEQ LGKKYNINPVSMDICKQEEK LGFLVAKQDLVISLLPYVLH PLVAKACITNKVNMVTASYI TPALKELEKSVEDAGITIIG E
Tissue Specificity Expressed in all 16 tissues examined with highest expression in the liver.
Function Bifunctional enzyme that catalyzes the first two steps in lysine degradation.
Protein Name Alpha-Aminoadipic Semialdehyde Synthase - Mitochondrial
Lkr/Sdh Includes - Lysine Ketoglutarate Reductase
Lkr
Lor - Saccharopine Dehydrogenase
Sdh
Database Links Reactome: R-HSA-71064
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Alpha-Aminoadipic Semialdehyde Synthase - Mitochondrial antibody
Anti-Lkr/Sdh Includes - Lysine Ketoglutarate Reductase antibody
Anti-Lkr antibody
Anti-Lor - Saccharopine Dehydrogenase antibody
Anti-Sdh antibody
Anti-AASS antibody

Information sourced from Uniprot.org

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