| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-AAK1 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
WB-1-3µg/mlELISA-antibody detection limit dilution 1:16000. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
AAK1 |
| Gene ID: |
22848 |
| Uniprot ID: |
AAK1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_055726.3 |
| Immunogen Sequence: |
GSPRTSQQNVYNPSE |
| Post Translational Modifications | Autophosphorylated. |
| Function | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Preferentially, may phosphorylate substrates on threonine residues. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity. (Microbial infection) By regulating clathrin-mediated endocytosis, AAK1 plays a role in the entry of hepatitis C virus as well as for the lifecycle of other viruses such as Ebola and Dengue. |
| Protein Name | Ap2-Associated Protein Kinase 1Adaptor-Associated Kinase 1 |
| Database Links | Reactome: R-HSA-8856825Reactome: R-HSA-8856828 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinMembraneClathrin-Coated PitPresynapseActive When Found In Clathrin-Coated Pits At The Plasma MembraneIn Neuronal CellsEnriched At Presynaptic TerminalsIn Non-Neuronal CellsEnriched At Leading Edge Of Migrating Cells |
| Alternative Antibody Names | Anti-Ap2-Associated Protein Kinase 1 antibodyAnti-Adaptor-Associated Kinase 1 antibodyAnti-AAK1 antibodyAnti-KIAA1048 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
