• Western blot analysis of extracts of various cell lines, using A-RAF antibody (STJ11103628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using A-RAF Rabbit monoclonal antibody (STJ11103628) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human liver using A-RAF rabbit monoclonal antibody (STJ11103628) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using A-RAF antibody (STJ11103628) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-A-RAF antibody (166-290) [S8MR] (STJ11103628)

SKU:
STJ11103628

£146.50 - £639.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-A-RAF (166-290) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ARAF
Gene ID: 369
Uniprot ID: ARAF_HUMAN
Immunogen Region: 166-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 166-290 of human A-RAF (P10398).
Immunogen Sequence: RQHEAPSNRPLNELLTPQGP SPRTQHCDPEHFPFPAPANA PLQRIRSTSTPNVHMVSTTA PMDSNLIQLTGQSFSTDAAG SRGGSDGTPRGSPSPASVSS GRKSPHSKSPAEQRERKSLA DDKKK
Tissue Specificity Predominantly in urogenital tissues.
Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation.
Protein Name Serine/Threonine-Protein Kinase A-Raf
Proto-Oncogene A-Raf
Proto-Oncogene A-Raf-1
Proto-Oncogene Pks
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Reactome: R-HSA-9726840
Reactome: R-HSA-9726842
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase A-Raf antibody
Anti-Proto-Oncogene A-Raf antibody
Anti-Proto-Oncogene A-Raf-1 antibody
Anti-Proto-Oncogene Pks antibody
Anti-ARAF antibody
Anti-ARAF1 antibody
Anti-PKS antibody
Anti-PKS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance