| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S8MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ARAF |
| Gene ID: | 369 |
| Uniprot ID: | ARAF_HUMAN |
| Immunogen Region: | 166-290 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 166-290 of human A-RAF (P10398). |
| Immunogen Sequence: | RQHEAPSNRPLNELLTPQGP SPRTQHCDPEHFPFPAPANA PLQRIRSTSTPNVHMVSTTA PMDSNLIQLTGQSFSTDAAG SRGGSDGTPRGSPSPASVSS GRKSPHSKSPAEQRERKSLA DDKKK |
| Tissue Specificity | Predominantly in urogenital tissues. |
| Post Translational Modifications | Dephosphorylation of Ser-214 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC).this relieves inactivation and stimulates kinase activity. |
| Function | Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade. Phosphorylates PFKFB2. Isoform 2: Serves as a positive regulator of myogenic differentiation by inducing cell cycle arrest, the expression of myogenin and other muscle-specific proteins, and myotube formation. |
| Protein Name | Serine/Threonine-Protein Kinase A-RafProto-Oncogene A-RafProto-Oncogene A-Raf-1Proto-Oncogene Pks |
| Database Links | Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5675221Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9656223Reactome: R-HSA-9726840Reactome: R-HSA-9726842 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase A-Raf antibodyAnti-Proto-Oncogene A-Raf antibodyAnti-Proto-Oncogene A-Raf-1 antibodyAnti-Proto-Oncogene Pks antibodyAnti-ARAF antibodyAnti-ARAF1 antibodyAnti-PKS antibodyAnti-PKS2 antibody |
Information sourced from Uniprot.org