Western blot analysis of extracts of various cell lines, using 5T4 Rabbit monoclonal antibody (STJ11103034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-5T4 antibody (321-420) [S4MR] (STJ11103034)

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STJ11103034
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TPBG
Gene ID: 7162
Uniprot ID: TPBG_HUMAN
Immunogen Region: 321-420
Specificity: A synthetic peptide corresponding to a sequence within amino acids 321-420 of human 5T4 (Q13641).
Immunogen Sequence: LTCAYPEKMRNRVLLELNSA DLDCDPILPPSLQTSYVFLG IVLALIGAIFLLVLYLNRKG IKKWMHNIRDACRDHMEGYH YRYEINADPRLTNLSSNSDV
Tissue Specificity Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
Post Translational Modifications Highly glycosylated.
Function May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Protein Name Trophoblast Glycoprotein
5t4 Oncofetal Antigen
5t4 Oncofetal Trophoblast Glycoprotein
5t4 Oncotrophoblast Glycoprotein
M6p1
Wnt-Activated Inhibitory Factor 1
Waif1
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Trophoblast Glycoprotein antibody
Anti-5t4 Oncofetal Antigen antibody
Anti-5t4 Oncofetal Trophoblast Glycoprotein antibody
Anti-5t4 Oncotrophoblast Glycoprotein antibody
Anti-M6p1 antibody
Anti-Wnt-Activated Inhibitory Factor 1 antibody
Anti-Waif1 antibody
Anti-TPBG antibody
Anti-5T4 antibody

Information sourced from Uniprot.org

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