• Western blot analysis of lysates from HUVEC cells, using Arachidonate 5 Lipoxygenase Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Arachidonate 5 Lipoxygenase Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-5-LO antibody (STJ91392)

SKU:
STJ91392

Current Stock:
Host: Rabbit
Applications: WB, IHC-P, ELISA
Reactivity: Human, Mouse, Rat
Short Description: Rabbit polyclonal anti-5-LO antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Specificity: 5-LO polyclonal antibody detects endogenous levels of 5-LO protein.
Uniprot ID: LOX5_HUMAN
Gene ID: 240
Gene Symbol: ALOX5
Immunogen: Synthesized peptide derived from human 5-LO around the non-phosphorylation site of S272.
Immunogen Region: 220-300 aa
Function Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells.
Protein Name Polyunsaturated Fatty Acid 5-Lipoxygenase
Arachidonate 5-Lipoxygenase
5-Lo
5-Lipoxygenase
Database Links Reactome: R-HSA-2142688
Reactome: R-HSA-2142691
Reactome: R-HSA-2142700
Reactome: R-HSA-6785807
Reactome: R-HSA-6798695
Reactome: R-HSA-9012546
Reactome: R-HSA-9018676
Reactome: R-HSA-9018682
Reactome: R-HSA-9018896
Reactome: R-HSA-9020265
Reactome: R-HSA-9023661
Reactome: R-HSA-9026286
Reactome: R-HSA-9026290
Reactome: R-HSA-9026403
Reactome: R-HSA-9027604
Cellular Localisation Cytoplasm
Nucleus Matrix
Nucleus Membrane
Peripheral Membrane Protein
Perinuclear Region
Cytosol
Nucleus Envelope
Nucleus Intermembrane Space
Shuttles Between Cytoplasm And Nucleus
Found Exclusively In The Nucleus
When Phosphorylated On Ser-272
Calcium Binding Promotes Translocation From The Cytosol And The Nuclear Matrix To The Nuclear Envelope And Membrane Association
Alternative Antibody Names Anti-Polyunsaturated Fatty Acid 5-Lipoxygenase antibody
Anti-Arachidonate 5-Lipoxygenase antibody
Anti-5-Lo antibody
Anti-5-Lipoxygenase antibody
Anti-ALOX5 antibody
Anti-LOG5 antibody

Information sourced from Uniprot.org

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