||WB, IHC-P, ELISA
||Human, Mouse, Rat
||Rabbit polyclonal anti-5-LO antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
||FOR RESEARCH USE ONLY (RUO).
||Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
||Store at-20°C, and avoid repeat freeze-thaw cycles.
||WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000
||5-LO polyclonal antibody detects endogenous levels of 5-LO protein.
||Synthesized peptide derived from human 5-LO around the non-phosphorylation site of S272.
| Function || Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. Also may play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells. |
| Protein Name || Polyunsaturated Fatty Acid 5-LipoxygenaseArachidonate 5-Lipoxygenase5-Lo5-Lipoxygenase |
| Database Links || Reactome: R-HSA-2142688Reactome: R-HSA-2142691Reactome: R-HSA-2142700Reactome: R-HSA-6785807Reactome: R-HSA-6798695Reactome: R-HSA-9012546Reactome: R-HSA-9018676Reactome: R-HSA-9018682Reactome: R-HSA-9018896Reactome: R-HSA-9020265Reactome: R-HSA-9023661Reactome: R-HSA-9026286Reactome: R-HSA-9026290Reactome: R-HSA-9026403Reactome: R-HSA-9027604 |
| Cellular Localisation || CytoplasmNucleus MatrixNucleus MembranePeripheral Membrane ProteinPerinuclear RegionCytosolNucleus EnvelopeNucleus Intermembrane SpaceShuttles Between Cytoplasm And NucleusFound Exclusively In The NucleusWhen Phosphorylated On Ser-272Calcium Binding Promotes Translocation From The Cytosol And The Nuclear Matrix To The Nuclear Envelope And Membrane Association |
| Alternative Antibody Names || Anti-Polyunsaturated Fatty Acid 5-Lipoxygenase antibodyAnti-Arachidonate 5-Lipoxygenase antibodyAnti-5-Lo antibodyAnti-5-Lipoxygenase antibodyAnti-ALOX5 antibodyAnti-LOG5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance