Host: | Rabbit |
Applications: | WB/IHC/IF/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-Polyunsaturated fatty acid 5-lipoxygenase (246-295 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Concentration: | 1 mg/mL |
Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200 |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | ALOX5 |
Gene ID: | 240 |
Uniprot ID: | LOX5_HUMAN |
Immunogen Region: | 246-295 aa |
Specificity: | 5-LO Polyclonal Antibody detects endogenous levels of 5-LO protein. |
Immunogen: | The antiserum was produced against synthesized peptide derived from the human Arachidonate 5 Lipoxygenase at the amino acid range 246-295 |
Function | Catalyzes the oxygenation of arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate) to 5-hydroperoxyeicosatetraenoate (5-HPETE) followed by the dehydration to 5,6- epoxyeicosatetraenoate (Leukotriene A4/LTA4), the first two steps in the biosynthesis of leukotrienes, which are potent mediators of inflammation. Also catalyzes the oxygenation of arachidonate into 8-hydroperoxyicosatetraenoate (8-HPETE) and 12-hydroperoxyicosatetraenoate (12-HPETE). Displays lipoxin synthase activity being able to convert (15S)-HETE into a conjugate tetraene. Although arachidonate is the preferred substrate, this enzyme can also metabolize oxidized fatty acids derived from arachidonate such as (15S)-HETE, eicosapentaenoate (EPA) such as (18R)- and (18S)-HEPE or docosahexaenoate (DHA) which lead to the formation of specialized pro-resolving mediators (SPM) lipoxin and resolvins E and D respectively, therefore it participates in anti-inflammatory responses. Oxidation of DHA directly inhibits endothelial cell proliferation and sprouting angiogenesis via peroxisome proliferator-activated receptor gamma (PPARgamma). It does not catalyze the oxygenation of linoleic acid and does not convert (5S)-HETE to lipoxin isomers. In addition to inflammatory processes, it participates in dendritic cell migration, wound healing through an antioxidant mechanism based on heme oxygenase-1 (HO-1) regulation expression, monocyte adhesion to the endothelium via ITGAM expression on monocytes. Moreover, it helps establish an adaptive humoral immunity by regulating primary resting B cells and follicular helper T cells and participates in the CD40-induced production of reactive oxygen species (ROS) after CD40 ligation in B cells through interaction with PIK3R1 that bridges ALOX5 with CD40. May also play a role in glucose homeostasis, regulation of insulin secretion and palmitic acid-induced insulin resistance via AMPK. Can regulate bone mineralization and fat cell differentiation increases in induced pluripotent stem cells. |
Protein Name | Polyunsaturated Fatty Acid 5-Lipoxygenase Arachidonate 5-Lipoxygenase 5-Lo 5-Lipoxygenase |
Database Links | Reactome: R-HSA-2142688 Reactome: R-HSA-2142691 Reactome: R-HSA-2142700 Reactome: R-HSA-6785807 Reactome: R-HSA-6798695 Reactome: R-HSA-9012546 Reactome: R-HSA-9018676 Reactome: R-HSA-9018682 Reactome: R-HSA-9018896 Reactome: R-HSA-9020265 Reactome: R-HSA-9023661 Reactome: R-HSA-9026286 Reactome: R-HSA-9026290 Reactome: R-HSA-9026403 Reactome: R-HSA-9027604 |
Cellular Localisation | Cytoplasm Nucleus Matrix Nucleus Membrane Peripheral Membrane Protein Perinuclear Region Cytosol Nucleus Envelope Nucleus Intermembrane Space Shuttles Between Cytoplasm And Nucleus Found Exclusively In The Nucleus When Phosphorylated On Ser-272 Calcium Binding Promotes Translocation From The Cytosol And The Nuclear Matrix To The Nuclear Envelope And Membrane Association |
Alternative Antibody Names | Anti-Polyunsaturated Fatty Acid 5-Lipoxygenase antibody Anti-Arachidonate 5-Lipoxygenase antibody Anti-5-Lo antibody Anti-5-Lipoxygenase antibody Anti-ALOX5 antibody Anti-LOG5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance