Anti-38261 antibody [R09-9M-1] (STJA0033051)

SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenPeptide
STJA0033051
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General Information

Short DescriptionMouse monoclonal anti-38261 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostMouse
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR09-9M-1
IsotypeIgG1k
ConjugationUnconjugated
PurificationProtein G purified
Dilution RangeWB 1:1000-1:5000
FormulationLiquid in PBS, Glycerol and BSA
Storage InstructionStore at-20°C. Avoid freeze/thaw cycles.

Target Information

Gene SymbolPOU5F1
Gene ID5460
Uniprot IDPO5F1_HUMAN
ImmunogenPeptide

Additional Info

Tissue Specificity Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
Post Translational Modifications Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
Protein Name Pou Domain - Class 5 - Transcription Factor 1
Octamer-Binding Protein 3
Oct-3
Octamer-Binding Protein 4
Oct-4
Octamer-Binding Transcription Factor 3
Otf-3
Database Links Reactome: R-HSA-2892245
Reactome: R-HSA-2892247
Reactome: R-HSA-452723
Reactome: R-HSA-9754189
Reactome: R-HSA-9823739
Reactome: R-HSA-9827857
Reactome: R-HSA-9834899
Cellular Localisation Cytoplasm
Nucleus
Expressed In A Diffuse And Slightly Punctuate Pattern
Colocalizes With Mapk8 And Mapk9 In The Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 5 - Transcription Factor 1 antibody
Anti-Octamer-Binding Protein 3 antibody
Anti-Oct-3 antibody
Anti-Octamer-Binding Protein 4 antibody
Anti-Oct-4 antibody
Anti-Octamer-Binding Transcription Factor 3 antibody
Anti-Otf-3 antibody
Anti-POU5F1 antibody
Anti-OCT3 antibody
Anti-OCT4 antibody
Anti-OTF3 antibody

Information sourced from Uniprot.org

Citations

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