alpha Actinin 4 Positive Control for STJ502086 peptide (STJ503628)
General Information
| Short Description | alpha Actinin 4 Positive Control for STJ502086 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:500 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | ACTN4 |
| Gene ID | 81 |
| Uniprot ID | ACTN4_HUMAN |
| Specificity | This is positive control is recommended for use in combination with alpha Actinin 4 antibody STJ502086. |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA. Association with IGSF8 regulates the immune synapse formation and is required for efficient T-cell activation. |
| Peptide Name | Alpha-Actinin-4Non-Muscle Alpha-Actinin 4 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-373753 |
| Cellular Localisation | NucleusCytoplasmCell JunctionCytoskeletonStress FiberPerinuclear RegionLocalized In Cytoplasmic Mrnp Granules Containing Untranslated MrnasExpressed In The Perinuclear Rim And Manchette Structure In Early Elongating Spermatids During SpermiogenesisNuclear Translocation Can Be Induced By The Pi3 Kinase Inhibitor Wortmannin Or By Cytochalasin DExclusively Localized In The Nucleus In A Limited Number Of Cell Lines (Breast Cancer Cell Line Mcf-7Oral Floor Cancer Imc-2And Bladder Cancer Ku-7) |
| Alternative Peptide Names | Alpha-Actinin-4 proteinNon-Muscle Alpha-Actinin 4 proteinACTN4 protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about alpha Actinin 4 Positive Control for STJ502086 peptide (STJ503628)
This is a positive control reagent intended for use in combination with the alpha Actinin 4 antibody STJ502086. It is designed to confirm antibody performance and validate Western blot results.
This positive control is validated for use in Western blot (WB) at a dilution of 1:500 with its corresponding antibody. No other applications are specified.
It is supplied as 100 uL ready-to-use in SDS-PAGE sample buffer (Laemmli buffer) containing Tris pH 6.8, 1% SDS, glycerol, and bromophenol blue as a tracking dye. The sample is reduced with 2% beta-mercaptoethanol, and the protein concentration is empirically adjusted to produce a clear band with an appropriate signal-to-noise ratio for STJ502086.
No additional preparation is required, as this positive control is provided ready-to-use in pre-reduced SDS-PAGE sample buffer. It can be loaded directly onto a gel without further dilution or processing.
This product should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain sample integrity.
This positive control corresponds to alpha Actinin 4, referenced under UniProt accession O43707. It is intended for use as a validated protein control when working with antibody STJ502086 in Western blot experiments.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.