ACK1 Blocking Peptide for STJ502083 peptide (STJ503620)
General Information
| Short Description | ACK1 Blocking Peptide for STJ502083 is synthetically produced from the 250-300 sequence and is suitable for use in western blot applications. |
| Applications | Immunodepletion/Immunocompetition |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Formulation | Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | TNK2 |
| Gene ID | 10188 |
| Uniprot ID | ACK1_HUMAN |
| Immunogen | Synthetic peptide taken within amino acid region 250-300 on ACK1 protein. |
| Immunogen Region | 250-300 |
| Specificity | This blocking peptide is recommended for use in combination with ACK1 antibody, STJ502083 |
Additional Info
| Tissue Specificity | The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages. |
| Post Translational Modifications | Autophosphorylation regulates kinase activity. Phosphorylation on Tyr-518 is required for interaction with SRC and is observed during association with clathrin-coated pits. Polyubiquitinated by NEDD4 and NEDD4L. Degradation can be induced by EGF and is lysosome-dependent. |
| Function | Non-receptor tyrosine-protein and serine/threonine-protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr-287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels. Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR. Phosphorylates WASP. |
| Peptide Name | Activated Cdc42 Kinase 1Ack-1Tyrosine Kinase Non-Receptor Protein 2 |
| Database Links | Reactome: R-HSA-9842663 |
| Cellular Localisation | Cell MembraneNucleusEndosomeCell JunctionAdherens JunctionCytoplasmic Vesicle MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic VesicleClathrin-Coated VesicleMembraneClathrin-Coated PitCytoplasmPerinuclear RegionCytosolThe Tyr-284 Phosphorylated Form Is Found Both In The Membrane And NucleusCo-Localizes With Egfr On EndosomesNuclear Translocation Is Cdc42-DependentDetected In Long Filamentous Cytosolic Structures Where It Co-Localizes With Ctps1 |
| Alternative Peptide Names | Activated Cdc42 Kinase 1 proteinAck-1 proteinTyrosine Kinase Non-Receptor Protein 2 proteinTNK2 proteinACK1 protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about ACK1 Blocking Peptide for STJ502083 peptide (STJ503620)
This is a blocking peptide intended for use in immunodepletion and immunocompetition experiments in combination with the ACK1 antibody STJ502083. It serves as a specificity control to confirm that signals detected by STJ502083 are due to binding of the antibody to its intended target.
This blocking peptide is a synthetic peptide derived from within the 250 to 300 amino acid region of the ACK1 protein, also known as TNK2 (UniProt Q07912).
This blocking peptide is recommended for immunodepletion and immunocompetition applications, where it is used alongside STJ502083 to verify antibody specificity.
This peptide is supplied in liquid form at 2.5 mg/ml in PBS, with the option to add up to 5% DMSO. Orders exceeding 1 mg can be supplied in lyophilized powder form or in a buffer of choice upon request.
This peptide should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain stability.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.