5HT1B Positive Control for STJ500005 peptide (STJ503614)
General Information
| Short Description | 5HT1B Positive Control for STJ500005 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | WB: 1:2, 500-1:5, 000 |
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Htr1b |
| Gene ID | 15551 |
| Uniprot ID | 5HT1B_MOUSE |
| Specificity | This is positive control is recommended for use in combination with 5HT1B antibody STJ500005. |
Additional Info
| Tissue Specificity | Predominantly expressed in striatum and Purkinje cells. |
| Post Translational Modifications | Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation. Palmitoylated. |
| Function | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for ergot alkaloid derivatives, various anxiolytic and antidepressant drugs and other psychoactive substances, such as lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. HTR1B is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission by inhibiting adenylate cyclase activity. Arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Regulates the release of 5-hydroxytryptamine, dopamine and acetylcholine in the brain, and thereby affects neural activity, nociceptive processing, pain perception, mood and behavior. Besides, plays a role in vasoconstriction of cerebral arteries. |
| Peptide Name | 5-Hydroxytryptamine Receptor 1b5-Ht-1b5-Ht1bSerotonin Receptor 1b |
| Database Links | Reactome: R-MMU-390666Reactome: -MMU-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Peptide Names | 5-Hydroxytryptamine Receptor 1b protein5-Ht-1b protein5-Ht1b proteinSerotonin Receptor 1b proteinHtr1b protein5ht1b protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about 5HT1B Positive Control for STJ500005 peptide (STJ503614)
This is a positive control reagent intended for use in combination with the 5HT1B antibody STJ500005. It provides a reference sample to confirm antibody performance and assay conditions in western blotting experiments.
This product is validated for use in western blot (WB) applications only, at a dilution range of 1:2,500 to 1:5,000.
It is supplied as 100 uL of a ready-to-use preparation in SDS-PAGE sample buffer (Laemmli buffer) containing Tris pH 6.8, 1% SDS, glycerol, and bromophenol blue as a tracking dye. The sample is pre-reduced with 2% beta-mercaptoethanol, so it can be loaded directly onto a gel without further preparation.
A specific protein concentration is not provided. The concentration has been empirically adjusted to produce a clear band with an acceptable signal-to-noise ratio when used with the corresponding antibody STJ500005.
This product should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
This positive control corresponds to the 5-hydroxytryptamine receptor 1B (5HT1B) protein, referenced under UniProt accession P28334. It is intended for research use only and is not suitable for diagnostic or therapeutic applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.