5HT1A Positive Control for STJ500002 peptide (STJ503612)
General Information
| Short Description | 5HT1A Positive Control for STJ500002 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Applications | WB |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Formulation | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Htr1a |
| Gene ID | 24473 |
| Uniprot ID | 5HT1A_RAT |
| Immunogen | Partially purified rat aquaporin 7 protein. |
| Specificity | This is positive control is recommended for use in combination with 5HT1A antibody STJ500002. |
Additional Info
| Tissue Specificity | Detected in hypothalamus, mesencephalon, amygdala, medulla, thalamus, septum and hippocampus. |
| Function | G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. HTR1A is coupled to G(i)/G(o) G alpha proteins and mediates inhibitory neurotransmission: signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Beta-arrestin family members regulate signaling by mediating both receptor desensitization and resensitization processes. |
| Peptide Name | 5-Hydroxytryptamine Receptor 1a5-Ht-1a5-Ht1aSerotonin Receptor 1a |
| Database Links | Reactome: R-RNO-390666 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendrite |
| Alternative Peptide Names | 5-Hydroxytryptamine Receptor 1a protein5-Ht-1a protein5-Ht1a proteinSerotonin Receptor 1a proteinHtr1a protein5ht1a protein |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about 5HT1A Positive Control for STJ500002 peptide (STJ503612)
This is a positive control reagent recommended for use in combination with the 5HT1A antibody STJ500002. It is intended to provide a confirmed positive signal when validating western blot results obtained with that specific antibody.
This positive control is prepared from partially purified rat aquaporin 7 protein.
It is supplied as 100 uL of a ready-to-use preparation in SDS-PAGE sample buffer (Laemmli buffer) containing Tris pH 6.8, 1% SDS, glycerol, and bromophenol blue as a tracking dye. The sample is reduced with 2% beta-mercaptoethanol, and the protein concentration is empirically adjusted to produce a clear band with an appropriate signal-to-noise ratio for use with its corresponding antibody.
This product is validated for use in western blot (WB) only. It is supplied in a ready-to-use format compatible with direct loading onto SDS-PAGE gels.
Store at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.