2019-nCoV Spike RBD protein (C-His) (STJP000019)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein is produced by HEK293Β cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2 (2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6ΓH
General Information
| Short Description | 2019-nCoV Spike RBD-C-His protein was developed in hek293 cells using the region Arg319-Phe541. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. or Supplied as a 0.22 Mu m filtered solution in PBS, pH 7.4. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA.Immobilized SARS-CoV-2 Spike RBD at 2 Mu g/mL (100 Mu L/well) can bind Human ACE-2 with a linear range of 0.001-2.96 ng/mL.|2.Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD P |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein is produced by HEK293Β cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2 (2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6ΓH |
| Immunogen Region | Arg319-Phe541 |
| Immunogen Sequence | RVQPTESIVRFPNITNLCPF GEVFNATRFASVYAWNRKRI SNCVADYSVLYNSASFSTFK CYGVSPTKLNDLCFTNVYAD SFVIRGDEVRQIAPGQTGKI ADYNYKLPDDFTGCVIAWNS NNLDSKVGGNYNYLYRLFRK SNLKPFERDISTEIYQAGST PCNGVEGFNCYFPLQSYGFQ PTNGVGYQPYRVVVLSFELL HAPATVCGPKKSTNLVKNKC VNF |
Additional Info
| Background | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-His Protein is produced by HEK293Β cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of SARS-COV-2 (2019-nCoV) Spike RBD-His (Accession #YP_009724390.1) fused with a 6ΓHis tag at the C-terminus. |
Information sourced from Uniprot.org