• Biotinylated COV2 S1 coating at 0. 1ug-well) binding with Human ACE2-Fc. The EC50 was determined to be 35 ng/ml
  • 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.
  • The purity of 2019-nCoV Spike S1 is greater than 95% as determined by SEC-HPLC.

2019 nCoV/COVID19 Spike protein S1 protein (Recombinant) {Biotin} (STJP000266)

SKU:
STJP000266

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-2019 nCoV/COVID19 Spike protein S1-protein was developed from hek293. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). NA
Immunogen Region: ECD
Immunogen: DNA sequence encoding the COVID-19 Spike S1 protein extracellular domain, [16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells. NA
Background Recombinant 2019-nCoV Spike S1 is a protein consisting of 680 amino acid residues with a calculated mW of 78kDa, due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE.

Information sourced from Uniprot.org

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