2019 nCoV/COVID19 Spike protein S1 protein (Recombinant) {Biotin} (STJP000266)
SPECIFICATIONS
HostHEK293
ConjugationBiotin
ImmunogenDNA sequence encoding the COVID-19 Spike S1 protein extracellular domain, [16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells. NA
General Information
| Short Description | Recombinant-2019 nCoV/COVID19 Spike protein S1-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Biotin |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Storage Instruction | The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by binding Human ACE2 in functional ELISA assay (see figure below). NA |
Target Information
| Immunogen | DNA sequence encoding the COVID-19 Spike S1 protein extracellular domain, [16-683] (accession # QHD43416.1) fused to aq C-terminal His AVI tag was expressed in HEK Cells. NA |
| Immunogen Region | ECD |
Additional Info
| Background | Recombinant 2019-nCoV Spike S1 is a protein consisting of 680 amino acid residues with a calculated mW of 78kDa, due to glycosylation migrates as an approximately 110 kDa protein on SDS-PAGE. |
Information sourced from Uniprot.org