• The recombinant RBD-hFc protein migrates as 70kDa due to glycosylation
  • The purity of 2019-nCoV Spike RBD Fc is greater than 95% as determined by SEC-HPLC.

2019 nCoV/COVID19 RBD protein (Recombinant) (mFc-Tag) (STJP000260)

SKU:
STJP000260

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Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-2019 nCoV/COVID19 RBD-mFc-Tag protein was developed from cho cells and has a target region of mFc-Tag. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 µm filtered 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by binding Human ACE2 in functional ELISA assay. NA
Immunogen Region: 319-514 aa
Immunogen: DNA sequence encoding the COVID-19 RBD protein domain [319-541] of NCOV spike S protein[accession# 6VSB_A ]including a C-terminal mouse IgG1 tag was expressed in CHO Cells. NA
Background Recombinant 2019-nCoV RBD protein is a protein consisting of 460 amino acid residues, due to glycosylation migrates as an approximately 70 kDa protein on SDS-PAGE.

Information sourced from Uniprot.org

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