2019 nCoV/COVID19 RBD protein (Recombinant) {Biotin} (STJP000261)

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STJP000261

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Host: CHO cells
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-2019 nCoV/COVID19 RBD-protein was developed from cho cells. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS pH 7.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was determined by immobilized human ACE2 Protein binding with biotinylated RBD in a functional ELISA assay, the linear was determined to be 10-100 ng/ml NA
Immunogen Region: 319-514 aa
Immunogen: DNA sequence encoding the COVID-19 spike RBD protein domain [319-541] accession #6VSB_A including a C-terminal His AVI tag was expressed in CHO Cells. NA
Background Recombinant 2019-nCoV RBD protein is a protein consisting of 250 amino acid residues, due to glycosylation migrates as an approximately 35kDa protein on SDS-PAGE.

Information sourced from Uniprot.org

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