| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | THAP1 Positive Control for STJ503247 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: | Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | SLC9A1 |
| Gene ID: | 6548 |
| Uniprot ID: | SL9A1_HUMAN |
| Specificity: | This is positive control is recommended for use in combination with THAP1 antibody STJ503247. |
| Immunogen: | Purified protein. |
| Tissue Specificity | Kidney and intestine. |
| Post Translational Modifications | O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1. Phosphorylation at Thr-779 increases SLC9A1 activity. Specifically dephosphorylated at Thr-779 by PPP3CA that negatively regulates SLC9A1 activity. Phosphorylation at Ser-648 by AKT1 reduces SLC9A1 binding to CALM1. Palmitoylated.may play a major role in SLC9A1 regulation. |
| Function | Electroneutral Na(+) /H(+) antiporter that extrudes Na(+) in exchange for external protons driven by the inward sodium ion chemical gradient, protecting cells from acidification that occurs from metabolism. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry. Plays a key role in maintening intracellular pH neutral and cell volume, and thus is important for cell growth, proliferation, migration and survival. In addition, can transport lithium Li(+) and functions also as a Na(+)/Li(+) antiporter. SLC9A1 also functions in membrane anchoring and organization of scaffolding complexes that coordinate signaling inputs. |
| Peptide Name | Sodium/Hydrogen Exchanger 1 Apnh Na(+/H(+ Antiporter - Amiloride-Sensitive Na(+/H(+ Exchanger 1 Nhe-1 Solute Carrier Family 9 Member 1 |
| Database Links | Reactome: R-HSA-2160916 Reactome: R-HSA-425986 |
| Cellular Localisation | Cell Membrane Multi-Pass Membrane Protein Basolateral Cell Membrane Localized Basolaterally In Every Epithelial Cell Except In The Choroid Plexus Where Slc9a1 Is Expressed Luminally |
| Alternative Peptide Names | Sodium/Hydrogen Exchanger 1 protein Apnh protein Na(+/H(+ Antiporter - Amiloride-Sensitive protein Na(+/H(+ Exchanger 1 protein Nhe-1 protein Solute Carrier Family 9 Member 1 protein SLC9A1 protein APNH1 protein NHE1 protein |
Information sourced from Uniprot.org