Mouse

Monoclonal Secondary Antibodies

Mouse Monoclonal Secondary Antibodies

Raised in mouse against a defined host-species immunoglobulin

Our mouse monoclonal secondary antibodies are produced from a single hybridoma clone raised against immunoglobulin from a target species, giving each antibody a defined, reproducible epitope. Our range currently includes 332 mouse monoclonal secondary antibodies, available unconjugated and across a range of enzyme, fluorophore and label conjugates.

How it works

Hybridoma-Based Production in a Mouse Host

Our mouse monoclonal secondary antibodies are generated by immunising mice with purified immunoglobulin from a target species, then fusing antibody-producing B cells from the mouse with myeloma cells to form a hybridoma. The resulting hybridoma is cultured to produce a secondary antibody that recognises a single, defined epitope on the target species' immunoglobulin, most commonly on the Fc region or as a heavy and light chain (H&L) conjugate. Because each clone descends from one parent hybridoma, every mouse monoclonal secondary in the range binds the same site on the target immunoglobulin from lot to lot.

Category benefits

Why use mouse monoclonal secondary antibodies?

✓ A large, established range
Our mouse monoclonal secondary range currently includes 332 products, giving a wide choice of target species and conjugates from a single, consistently produced hybridoma-derived line.
✓ A single, defined epitope
Because each mouse monoclonal secondary descends from one parent hybridoma clone, it binds the same site on the target immunoglobulin every time, supporting consistent detection across repeat experiments and lots.
✓ Broad conjugation options
Our mouse monoclonal secondaries are available unconjugated and across a wide set of labels, including HRP, biotin, FITC, gold, AMCA, TRITC, the Cy-series (Cy3, Cy5, Cy5.5, Cy7) and Alexa Fluor dyes spanning 350 to 790 nm, so a single clone can be matched to the detection method in use.
✓ Flexible pack sizes and concentrations
Products in the range are supplied at concentrations including 1 mg/mL and 2 mg/mL, in sizes from 50 µg and 100 µL trial packs up to 1 mL and 1 mg formats, so quantity can be matched to the scale of the experiment.
Where they're used

Typical applications

✓ Western blotting
HRP and biotin-conjugated mouse monoclonal secondaries detect the primary antibody bound to a target protein on the membrane, with single-epitope binding supporting consistent band detection across repeat runs.
✓ Immunohistochemistry and immunocytochemistry
Mouse monoclonal secondaries visualise a primary antibody bound in tissue sections or fixed cells, supporting expression and localisation studies.
✓ Immunofluorescence and flow cytometry
Fluorophore-conjugated mouse monoclonal secondaries, including Alexa Fluor and Cy-series dyes, detect unconjugated primary antibodies bound to surface or intracellular targets.
✓ ELISA and immunoprecipitation
Enzyme-conjugated mouse monoclonal secondaries support colorimetric or chemiluminescent detection in ELISA, and can be used to detect a primary antibody-antigen complex pulled down from a lysate.

Need help choosing a mouse secondary antibody?

Contact our technical support team for help matching a secondary antibody to your primary's host species and your detection method. Each product page includes a downloadable datasheet and SDS, with dispatch on overnight priority shipping with gel ice packs.

Looking for a different host?

Browse our full range of monoclonal secondary antibodies by host species.

 View all monoclonal secondary antibodies
240 of 332 Items
0 selected
!
Well-cited Academic Publications
KO-Validated 700+ Lines
Technical Support Expert Scientific Team
1-Year Guarantee Antibodies & Proteins