Conjugation

Conjugated Detection Reagents

Conjugated Secondary Antibodies

High-quality conjugates for fluorescence, chromogenic, chemiluminescent and electron microscopy detection

Our conjugated secondary antibody range includes AP, Biotin, DyLight, Alexa Fluor, FITC, Gold and HRP formats, validated for western blot, immunohistochemistry, immunofluorescence, ELISA and immunoelectron microscopy. With broad reactivity across chicken, donkey, goat and other host species, you can build a detection panel around your primary antibody.

Browse conjugated secondary antibodies View all secondary antibodies

How it works

Conjugated secondary antibodies for every assay

Secondary antibodies are raised against the host species of your primary antibody and are conjugated to a label for detection. Our range covers the most popular conjugates: HRP and AP for chromogenic and chemiluminescent detection; FITC, Alexa Fluor® and DyLight® dyes for fluorescence; biotin for signal amplification with streptavidin systems; and gold particles for immunoelectron microscopy. All products are validated for their stated applications and supplied with lot-specific data.

Category benefits

Why choose our conjugated secondary antibodies?

✓	Wide conjugate selection Choose from AP, Biotin, DyLight, Alexa Fluor, FITC, Gold and HRP to match your detection instrument, multiplexing needs and sample type.
✓	Validated across core techniques Products are validated for WB, IHC, ICC, IF, ELISA and immunoelectron microscopy, with application-specific recommendations on each product page.
✓	Broad species reactivity Our catalogue covers reactivity against chicken, goat, human, mouse, rabbit and more, with highly cross-adsorbed options for reduced background.
✓	Reliable, lot-to-lot consistency All conjugates are produced under strict quality control, with each lot tested for activity, specificity and titre before release.

Choose by conjugate

Conjugated secondary antibody categories

Browse our range by conjugate type: AP, Biotin, DyLight, Alexa Fluor, FITC, Gold, and HRP. Choose a category below to explore that part of the catalogue.

Enzyme AP Alkaline phosphatase conjugates for chromogenic and chemiluminescent detection.

Browse AP →

Affinity Tag Biotin Biotinylated secondary antibodies for signal amplification with streptavidin systems.

Browse Biotin →

Fluorescence DyLight DyLight® dyes (405, 488, 550, 633) for bright, photostable multiplex detection.

Browse DyLight →

Fluorescence Alexa Fluor Bright, photostable Alexa Fluor® dyes (488, 555, 568, 594, 633, 647) for IF and flow cytometry.

Browse Alexa Fluor →

Fluorescence FITC Fluorescein isothiocyanate conjugates for green-channel fluorescence detection.

Browse FITC →

Electron Microscopy Gold Gold particle conjugates for immunoelectron microscopy and blotting applications.

Browse Gold →

Enzyme HRP Horseradish peroxidase conjugates for chromogenic (DAB, TMB) and chemiluminescent (ECL) detection.

Browse HRP →

Where they're used

Typical applications

✓	Western blot HRP and AP conjugates are the standard for chemiluminescent and chromogenic detection, while fluorescent conjugates enable multiplex western blotting.
✓	Immunohistochemistry / Immunocytochemistry Enzyme conjugates (HRP, AP) for brightfield IHC; fluorescent conjugates (FITC, Alexa Fluor, DyLight) for multiplex IF; biotinylated options for amplification.
✓	ELISA HRP, AP and biotin conjugates are widely used as detection antibodies in sandwich and indirect ELISA formats.
✓	Immunofluorescence, flow cytometry & immunoelectron microscopy FITC, Alexa Fluor® and DyLight® conjugates are ideal for IF and flow cytometry; gold conjugates are used for immunoelectron microscopy and blotting.

Not sure which conjugate fits your assay?

Our technical team can help match a conjugated secondary antibody to your primary antibody host, target species and detection method. Get in touch for support choosing the right product.