Signal Transduction | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using eIF4EBP1 antibody (STJ29842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF4EBP1 antibody (50-118) (STJ29842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis using 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein (1).

Anti-EIF4EBP1 antibody [11G12C11] (STJ97805)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis using 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein (1) and A431 cell lysate (2).

Anti-EIF4EBP1 antibody [4B6G10] (STJ97804)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Anti-EIF4EBP1 antibody [9E12D9] (STJ97803)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Immunofluorescence analysis of HeLa cells, using eIF4G Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4G1 antibody (1074-1123 aa) (STJ92883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of HeLa cells, using EIF4G1 antibody (STJ29690) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EIF4G1 antibody (400-500) (STJ29690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Western blot analysis of HeLa, using EIF4G1 Rabbit polyclonal antibody (STJ23520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-EIF4G1 antibody (550-650) (STJ23520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Anti-EIF4G1 antibody (551-650 aa) [SRM] (STJ11107173)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

Choose Options

Immunoprecipitation analysis of 150 Mu g extracts of A549 cells using 3 Mu g ESE1/ESE1/ELF3 antibody (STJ115450). Western blot was performed from the immunoprecipitate using ESE1/ESE1/ELF3 antibody (STJ115450) at a dilution of 1:500. ____Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-ELF3 antibody (1-280) (STJ115450)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded rat lung using ESE1/ELF3 Rabbit polyclonal antibody (STJ28454) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-ELF3 antibody (1-280) (STJ28454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ HepG5 with 1 Mu g ESE1 (STJ11102304) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of ESE1 in representative gene loci (MARK4) , as shown in figure.

Anti-ELF3 antibody (1-371) [S4MR] (STJ11102304)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Anti-ELF3 antibody (N-Term) (STJA0005721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

Choose Options

Western blot analysis of extracts of various cell lines, using ELK1 antibody (STJ23526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ELK1 antibody (201-297) (STJ23526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g ELK1 antibody (STJ11101584). Western blot was performed from the immunoprecipitate using ELK1 antibody (STJ11101584) at a dilution of 1:1000.

Anti-ELK1 antibody (329-428) [S4MR] (STJ11101584)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of HeLa cells using Elk-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ELK1 antibody (351-400 aa) (STJ92892)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ELK1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-ELK1 antibody (408-428 aa) [R06-5J2] (STJA0011273)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ChIP

Reactivity :

Human

£246.50

Choose Options

Western blot analysis using Elk1 monoclonal antibody against truncated ELK1 recombinant protein (1) and K562 cell lysate (2).

Anti-ELK1 antibody [3H6D12/4H9C8/4H9F1] (STJ98017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Anti-ELK1 antibody [7E10D5/7E10E8] (STJ98018)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis of extracts from NIH-3T3 cells, using Elmo1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-ELMO1 antibody (22-71 aa) (STJ96395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of various lysates using ELMO1 Rabbit polyclonal antibody (STJ117030) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ELMO1 antibody (527-727) (STJ117030)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human appendix using ELMO1 Rabbit monoclonal antibody (STJ11102280) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-ELMO1 antibody (628-727) [S0MR] (STJ11102280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates using ELMO2 Rabbit polyclonal antibody (STJ115730) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ELMO2 antibody (42-120) (STJ115730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EMC3 antibody (54-104 aa) (STJ194535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Confocal immunofluorescence analysis of U-2OS cells using Emerin/Emerin/EMD antibody (STJ115860) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EMD antibody (1-220) (STJ115860)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Confocal immunofluorescence analysis of U-2 OS cells using Emerin/EMD Rabbit polyclonal antibody (STJ23533) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-EMD antibody (1-220) (STJ23533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Confocal imaging of HeLa cells using Emerin/EMD Rabbit monoclonal antibody (STJ11102013, dilution 1:100) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-EMD antibody (150-254) [S3MR] (STJ11102013)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-EMD antibody (151-201 aa) (STJ194286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-Emerin antibody [4G5] (STJ16101736)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F

Reactivity :

Human

£702.50

Choose Options

Western blot analysis of various lysates using EMR2 Rabbit polyclonal antibody (STJ116490) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-EMR2 antibody (300-500) (STJ116490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

Choose Options

Anti-Endothelin B receptor antibody (C-Term) (STJ13100125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

Choose Options

Immunohistochemistry of human ovarian medulla with Anti-ENSG00000285188 primary antibody (STJ502317) at 1: dilution in buffer.

Anti-ENSG00000285188 antibody (C-Term) (STJ502317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£466.50

Choose Options

Anti-ENSG00000285188 antibody (C-Term) {Biotin} (STJ502318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Anti-ENSG00000285188 antibody (C-Term) {FITC} (STJ502319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using EP300 rabbit polyclonal antibody (STJ114983) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-EP300 antibody (1-270) (STJ114983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of EP300 in paraffin-embedded mouse spleen tissue using EP300 Rabbit polyclonal antibody (STJ11105314) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-EP300 antibody (940-1030) (STJ11105314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of Hela cell. 1, p300 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). p53 monoclonal antibody (6C4) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-EP300 antibody (Internal) (STJ97645)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis using p300 monoclonal antibody against truncated p300-His recombinant protein (1).

Anti-EP300 antibody [7D8A6] (STJ98299)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Western blot analysis of extracts of various cell lines, using EPAS1/HIF2 Alpha antibody (STJ29691) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-EPAS1 antibody (678-745) (STJ29691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of human-stomach tissue. 1, EPAS-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-EPAS1 antibody (STJ96690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of 293T, 823 cells using Epigen Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-EPGN antibody (11-60 aa) (STJ97351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of 22RV1 cells using EphA2 Polyclonal Antibody diluted at 1：1000

Anti-EPHA2 antibody (131-180 aa) (STJ92940)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of extracts of various cell lines, using EPHA2 antibody (STJ29263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-EPHA2 antibody (280-530) (STJ29263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-EPHA2 antibody (Extracellular region) [M049] (STJA0005401)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB-Nat/ICC/IP

Reactivity :

Human

£390.50

Choose Options

Anti-EPHA2 antibody (Extracellular region) [M060] (STJA0005402)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB-Nat/ICC

Reactivity :

Human

£390.50

Choose Options

Anti-EPHA2 antibody (Extracellular region) [M088] (STJA0005403)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IP

Reactivity :

Human

£390.50

Choose Options

Western blot analysis using EphA2 monoclonal antibody against NIH/3T3 cell lysate.

Anti-EPHA2 antibody [1B3C7] (STJ98024)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

Choose Options

Anti-Epidermal Growth Factor Receptor antibody [EGFR.113] (STJ16101734)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£1,389.50

Choose Options

Immunohistochemistry analysis of EPN1 in paraffin-embedded Mouse kidney using EPN1 Rabbit polyclonal antibody (STJ119738) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EPN1 antibody (130-180) (STJ119738)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options