Signal Transduction | Research Area | St John's Laboratory

Immunohistochemical analysis of paraffin-embedded Skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-COL3A1 antibody (100-200 aa) [PT0118] (STJ196955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 Rabbit polyclonal antibody (STJ23192) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL3A1 antibody (1201-1301) (STJ23192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunocytochemistry analysis of Collagen III (green) in Hela using Collagen III antibody, and DAPI (blue)

Anti-COL3A1 antibody (201-400 aa) [R06-4E1] (STJA0012676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Immunofluorescence analysis of HeLa cells, using Collagen III Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL3A1 antibody (81-130 aa) (STJ92388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-COL3A1 antibody [ABT192R] (STJ197075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-COL3A1 antibody [Q76] (STJ97025)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of mouse-musle cells using COL4A1 Polyclonal Antibody diluted at 1：500

Anti-COL4A1 antibody (11-60 aa) (STJ92389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of Hela MCF-7 U2OS 293T KB 293T VEC cells using Collagen IV Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000

Anti-COL4A1 antibody (1428-1443 aa) (STJ98599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of U-87 MG cells, using COL4A1 Antibody (STJ112627) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-COL4A1 antibody (1445-1669) (STJ112627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-ype IV (ABT165) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 161kDaObserved band size: 180, 50, 37kDa

Anti-COL4A1 antibody (1600-1669 aa) [ABT165] (STJ197221)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse

£174.00 - £252.00

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Western blot analysis of HeLa, using COL4A2 Rabbit polyclonal antibody (STJ29971) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-COL4A2 antibody (1486-1712) (STJ29971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1：2000

Anti-COL4A2 antibody (151-200 aa) (STJ92390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of mouse kidney, using COL4A3 antibody (STJ118787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-COL4A3 antibody (1555-1669) (STJ118787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of COS7 cells, using Collagen IV alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A3 antibody (801-850 aa) (STJ92391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293F, using COL4A4 antibody (STJ11104960) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-COL4A4 antibody (1573-1690 aa) (STJ11104960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of COS7 cells, using Collagen IV alpha4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A4 antibody (541-590 aa) (STJ92392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-COL4A4/COL4A6/COL4A2/COL4A1 antibody (1573-1690 aa) [SRM] (STJ11107165)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-COL4A5 antibody (1462-1691 aa) (STJ11106204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using COL4A5 antibody (STJ111851) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-COL4A5 antibody (1462-1691) (STJ111851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A5 antibody (21-70 aa) (STJ92393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of Saos-2, using COL5A1 Rabbit polyclonal antibody (STJ23194) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-COL5A1 antibody (300-400) (STJ23194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various cells using COL5A1 Polyclonal Antibody

Anti-COL5A1 antibody (301-350 aa) (STJ92395)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HepG2 cells, using Collagen V alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL5A2 antibody (1-50 aa) (STJ92397)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HepG2 cells using COL5A2 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-COL5A2 antibody (1-50 aa) (STJ96898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using COL5A3 Polyclonal Antibody

Anti-COL5A3 antibody (221-270 aa) (STJ92398)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Collagen VI/Collagen VI/COL6A1 antibody (STJ111622) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-COL6A1 antibody (19-250) (STJ111622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human heart tissue, using Collagen VI alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL6A1 antibody (191-240 aa) (STJ92399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Collagen VI/COL6A1 rabbit monoclonal antibody (STJ11102536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-COL6A1 antibody (37-235) [S6MR] (STJ11102536)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of K562 cells using COL6A2 Polyclonal Antibody

Anti-COL6A2 antibody (691-740 aa) (STJ92400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Monkey

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using COL6A2 antibody (STJ23195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-COL6A2 antibody (720-1019) (STJ23195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells, using Collagen VI alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL6A3 antibody (2261-2310 aa) (STJ92401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using COL9A1 Rabbit polyclonal antibody antibody (STJ28646) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-COL9A1 antibody (20-270) (STJ28646)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Western blot analysis of HepG2 cells using COL9A1 Polyclonal Antibody

Anti-COL9A1 antibody (390-470 aa) (STJ92403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-COL9A2 antibody (540-620 aa) (STJ190272)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Rat liver, using COL9A2 Rabbit polyclonal antibody (STJ119576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-COL9A2 antibody (600-689) (STJ119576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells, using Collagen IX alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL9A3 antibody (321-370 aa) (STJ92404)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of paraffin-embedded mouse heart using COL9A3 antibody (STJ111840) at dilution of 1:100 (40x lens).

Anti-COL9A3 antibody (445-684) (STJ111840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.

Anti-Collagen 1, alpha 1 propeptide antibody (STJA0003599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Feline/Finch/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus

£454.50

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Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (STJA0003600, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Anti-Collagen 1, alpha 1 telopeptide antibody (STJA0003600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Sheep/Bovine/Canine/Chicken/Feline/Finch/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Vole/Xenopus

£454.50

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Anti-Collagen I antibody [SM0490] (STJA0010490)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Collagen II antibody [SM5168] (STJA0005168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715) at a dilution of 1:1000.

Anti-Collagen III alpha 1/COL3A1 antibody (150-400) [S5MR] (STJ11102715)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Collagen III antibody [SM0439] (STJA0010439)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-Collagen IV antibody (1555-1669 aa) [SRM] (STJ11107350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Human glomus tumor stained with anti-Collagen IV using peroxidase-conjugate and DAB chromogen. Note basement membrane staining of around individual cells.

Anti-Collagen IV antibody [ZM177] (STJ180502)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£151.50 - £497.50

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Anti-Connexin 43 antibody (300-382 aa) [SRM] (STJ11106966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of A-549 cells using COPS2 antibody (STJ29117).

Anti-COPS2 antibody (1-310) (STJ29117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-COPS2 antibody (181-230 aa) (STJ96104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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