Programmed Cell Death | Research Area | St John's Laboratory

Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded mouse pancreas using Flotillin 1 Rabbit polyclonal antibody (STJ11104576) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-FLOT1 antibody (128-427) (STJ11104576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Flotillin 1 antibody (STJ27976). Western blot was performed from the immunoprecipitate using Flotillin 1 antibody (STJ27976) at a dilution of 1:1000.

Anti-FLOT1 antibody (155-427) (STJ27976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of Flotillin 1 in paraffin-embedded human tonsil tissue using Flotillin 1 Rabbit monoclonal antibody (STJ11101835) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-FLOT1 antibody (328-427) [S5MR] (STJ11101835)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-FLOT1 antibody (STJ194764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Mouse heart using FLOT2 Rabbit polyclonal antibody (STJ11104327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-FLOT2 antibody (239-428) (STJ11104327)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of various cells using Flotillin-2 Polyclonal Antibody

Anti-FLOT2 antibody (95-144 aa) (STJ93092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using FNTA antibody (STJ111424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-FNTA antibody (280-379) (STJ111424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-FNTA antibody (STJA0008051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Endogenous CDH1 detected with anti-CDH1 at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-FZR1 antibody (601 to 701) (STJ140099)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Endogenous CDH1 detected at 1:500 dilution lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-FZR1 antibody (750 to 850) (STJ140027)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Immunohistochemistry analysis of paraffin-embedded human liver cancer using GAS2 antibody (STJ23749) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-GAS2 antibody (1-100) (STJ23749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from Rat liver, using GAS2 Rabbit monoclonal antibody (STJ11105146) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GAS2 antibody (1-100) [S5146RM] (STJ11105146)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GAS2 antibody (STJ190841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Human tonsil stained with anti-Granzyme B antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic granular staining of cytotoxic T-cells.

Anti-Granzyme B antibody (73-187aa) [ZM66] (STJ180310)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £549.50

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Anti-Granzyme B antibody (STJ16101330)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£653.50 - £1,177.50

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Anti-Granzyme B antibody [11F1] (STJ16101744)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£877.50

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Anti-Granzyme B antibody [GrB-7] (STJ16100752)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/ELISA/IHC-P

Reactivity :

Human

£531.50

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Anti-Granzyme B antibody [SM0403] (STJA0010403)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Anti-Granzyme B antibody [SM0404] (STJA0010404)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Rat

£170.50 - £233.50

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Immunofluorescence analysis of un-treated (left) A549 and UV treated (right) A549 cell. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min.

Anti-GSDMD antibody (1-100) (STJA0010253)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit monoclonal antibody (STJ11105210) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 10 ng per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. western blot samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS

Anti-GSDMD antibody (276-484) [S5210RM] (STJ11105210)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunoprecipitation analysis of 300 Mu g extracts of Jurkat cells using 3 Mu g GSDMD antibody (STJ11100237). Western blot was performed from the immunoprecipitate using GSDMD antibody (STJ11100237) at a dilution of 1:500.

Anti-GSDMD antibody (346-484) (STJ11100237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using GSDMD Rabbit polyclonal antibody (STJ119438) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSDMD antibody (346-484) (STJ119438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from THP-1 cells, using GSDMD Rabbit monoclonal antibody (STJ11103428) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight and LPS (1 Mu g/ml) at 37 °C for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-GSDMD antibody (346-484) [S8MR] (STJ11103428)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Anti-GSDMD antibody (Full Length + N-Term) (STJ11105919)

Clonality :

Polyclonal

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-GSDMD antibody (Full Length + N-Term) (STJ11106595)

Clonality :

Polyclonal

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of GSDMD in Jurkat lysates using GSDMD antibody.

Anti-GSDMD antibody (Full-length) [R06-5C5] (STJA0013051)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£264.50

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Anti-GSDME antibody (1-100) (STJA0010252)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of DFNA5/GSDME in U251MG, U2OS, HepG2, Hela lysates using GSDME antibody.

Anti-GSDME antibody (1-270 aa) [R07-5I1] (STJA0011231)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£246.50

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Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-GSDME antibody (200-280 aa) (STJ96411)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of extracts of Jurkat cells, using DFNA5/GSDME antibody (STJ29568) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GSDME antibody (271-496) (STJ29568)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from 1) Mouse-brain, 2) Hela cells treated by UV 15min, 3) HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody HRP goat anti rabbit (STJ99666) was diluted at 1:10000

Anti-GSDME antibody (400-496) (STJA0010251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of Gelsolin in paraffin-embedded human tonsil using Gelsolin Rabbit polyclonal antibody (STJ11104659) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (28-782) (STJ11104659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using Gelsolin Rabbit polyclonal antibody (STJ11101188) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-GSN antibody (282-438) (STJ11101188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded mouse brain using Gelsolin Rabbit monoclonal antibody (STJ11101830) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSN antibody (400-500) [S0MR] (STJ11101830)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western Blot analysis of varius cell lysis. primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-GSN antibody (STJ192165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry of rat spleen with Anti-GZMB primary antibody (STJ501289) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-GZMB antibody (1-50) (STJ501289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Rat

£427.50

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Anti-GZMB antibody (1-50) {Biotin} (STJ501291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Rat

£528.50

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Anti-GZMB antibody (1-50) {FITC} (STJ501292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Monkey/Rat

£528.50

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Anti-GZMB antibody (100-200 aa) [PT0564] (STJ196705)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of mouse spleen cells using Granzyme B Rabbit polyclonal antibody (STJ26211) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GZMB antibody (100-200) (STJ26211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry of baboon spleen with Anti-GZMB primary antibody (STJ501290) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-GZMB antibody (50-100) (STJ501290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£427.50

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Anti-GZMB antibody (50-100) {Biotin} (STJ501293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Anti-GZMB antibody (50-100) {FITC} (STJ501294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey

£528.50

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Western blot analysis of mouse-lung lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-GZMB antibody (81-130 aa) (STJ99635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Histone 1.0 Polyclonal Antibody diluted at 1：2000

Anti-H1-0 antibody (71-120 aa) (STJ93509)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using HIST1H1A Rabbit polyclonal antibody (STJ11103985) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-H1-1 antibody (116-168) (STJ11103985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-H1-1 antibody (49-99 aa) (STJ195717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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