Neuronal System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using SNAP25 Rabbit monoclonal antibody (STJ11101797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SNAP25 antibody (107-206) [S7MR] (STJ11101797)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using SNAP25 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-SNAP25 antibody (151-200 aa) (STJ95717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunocytochemistry analysis of SNAP25 (green) in Hela using SNAP25 antibody, and DAPI (blue).

Anti-SNAP25 antibody (193-206 aa) [R07-1D7] (STJA0013092)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Western blot analysis of extracts of various cell lines, using Src antibody (STJ11101656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SRC antibody (1-100) [S6MR] (STJ11101656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using Src Rabbit polyclonal antibody (STJ113306) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SRC antibody (1-80) (STJ113306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SRC antibody (200-300) (STJ503200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SRC antibody (200-300) {Biotin} (STJ503202)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SRC antibody (200-300) {FITC} (STJ503203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of rat-lung tissue. 1, c-Src Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-SRC antibody (360-440 aa) (STJ92501)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-SRC primary antibody (STJ503201) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SRC antibody (400-500) (STJ503201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SRC antibody (400-500) {Biotin} (STJ503204)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SRC antibody (400-500) {FITC} (STJ503205)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Src (PTR2316) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HUVEC Lane 3: A431Predicted band size: 60kDaObserved band size: 60kDa

Anti-SRC antibody (400-536 aa) [PTR2316] (STJ193243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from SH-SY5Y cells, using Src Rabbit polyclonal antibody (STJ25689) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SRC antibody (410-536) (STJ25689)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using Src polyclonal antibody (STJ11100197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SRC antibody (430-530) (STJ11100197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SRC antibody (N-Term) [M259] (STJA0005601)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Src mouse Monoclonal antibody diluted at 1:200.

Anti-SRC antibody [Mix] (STJ196656)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-Stargazin antibody (STJ13100088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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Anti-Stargazin antibody (STJ13100342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£331.50

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Western blot analysis of extracts of various cell lines, using STX1A rabbit polyclonal antibody (STJ110240) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STX1A antibody (1-100) (STJ110240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of mouse brain using STX1A Rabbit monoclonal antibody (STJ11102975) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-STX1A antibody (1-100) [S5MR] (STJ11102975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of C6 cells using STX1A Polyclonal Antibody (STJ27540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-STX1A antibody (1-288) (STJ27540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Syntaxin 1 Polyclonal Antibody

Anti-STX1A antibody (1-50 aa) (STJ95858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of K562 cells using Syntaxin 1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-STX1A antibody (31-80 aa) (STJ96805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat cortical lysate showing specific immunolabeling of the ~ 36 kDa syntaxin 1A protein.

Anti-STX1A antibody [SP8] (STJA0003793)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Rat/Pig/Hamster

£454.50

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Immunofluorescence analysis of NIH/3T3 cells, using MUNC-18a Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-STXBP1 antibody (279-328 aa) (STJ96187)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using STXBP1 antibody (STJ27373) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-STXBP1 antibody (295-594) (STJ27373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Rat

£112.50 - £524.50

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Western blot analysis of extracts of HeLa cells, using STXBP1 rabbit monoclonal antibody (STJ11102476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-STXBP1 antibody (400-500) [S6MR] (STJ11102476)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using SYN1 Rabbit polyclonal antibody (STJ119488) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-SYN1 antibody (1-705) (STJ119488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Western blot analysis of NIH-3T3 cells using Synapsin I Polyclonal Antibody

Anti-SYN1 antibody (26-75 aa) (STJ95847)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Synapsin I Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-SYN1 antibody (3-52 aa) (STJ95848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of rat brain using SYN1 Rabbit polyclonal antibody (STJ11105704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SYN1 antibody (400-500) (STJ11105704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 293T 3T3 COLO using SYN1 antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-SYN1 antibody (Internal) (STJ97690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SYN2 antibody (370-450 aa) (STJ190171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SYN3 antibody (30-120) (STJ11101491)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Anti-SYN3 antibody (530-580 aa) (STJ191485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Synapsin 1 antibody (595-645 aa) (STJ13100349)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Anti-Synapsin 2 antibody (420-470) (STJ13100350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/WB

Reactivity :

Human/Rat/Mouse

£331.50

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Confocal imaging of paraffin-embedded Rat brain using Synapsin II Rabbit monoclonal antibody (STJ11103035, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Synapsin II antibody (200-300) [S5MR] (STJ11103035)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-Synapsin-1 antibody (401-500 aa) [SRM] (STJ11107349)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Anti-Synaptotagmin2 antibody (STJ13100322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Marmoset

£331.50

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Western blot analysis of lysates from Mouse brain using SYT1 Rabbit polyclonal antibody (STJ11105483) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-SYT1 antibody (1-100) (STJ11105483)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Anti-SYT1 antibody (1-60) (STJ503197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SYT1 antibody (1-60) {Biotin} (STJ503198)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SYT1 antibody (1-60) {FITC} (STJ503199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunofluorescence analysis of mouse eye using SYT1 Rabbit polyclonal antibody (STJ25763) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SYT1 antibody (84-157) (STJ25763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis using Synaptotagmin 1 monoclonal antibody against truncated Trx-Synaptotagmin 1 recombinant protein (1).

Anti-SYT1 antibody [8G11B10] (STJ98407)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of 293 cells using Synaptotagmin X Polyclonal Antibody

Anti-SYT10 antibody (380-460 aa) (STJ95851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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