Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using PKA RII Alpha (PRKAR2A) Rabbit monoclonal antibody (STJ11101964) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKAR2A antibody (312-404) [S4MR] (STJ11101964)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of 293 cells using PKA II Alpha reg Polyclonal Antibody

Anti-PRKAR2A antibody (41-90 aa) (STJ95109)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded rat ovary using PRKAR2B Rabbit monoclonal antibody (STJ11103108) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PRKAR2B antibody (319-418) [S8MR] (STJ11103108)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using PKA II Beta reg Polyclonal Antibody

Anti-PRKAR2B antibody (79-128 aa) (STJ95110)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates, using PKC alpha Rabbit monoclonal antibody (STJ11105041) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRKCA antibody (200-300 aa) [S5041RM] (STJ11105041)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-PKC (PTR1190) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 70kDaObserved band size: 80kDa

Anti-PRKCA antibody (450-550) [PTR1190] (STJA0006229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Rabbit/Bovine

£49.00 - £261.00

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Western blot analysis of various lysates, using PKC alpha Rabbit polyclonal antibody (STJ115305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRKCA antibody (573-672) (STJ115305)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using PKC alpha antibody (STJ25125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-PRKCA antibody (573-672) (STJ25125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PKC alpha antibody (STJ112958). Western blot was performed from the immunoprecipitate using PKC alpha antibody (STJ112958) at a dilution of 1:1000.

Anti-PRKCA antibody (573-672) [S8MR] (STJ112958)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of NIH-3T3 cells using PKC Alpha Polyclonal Antibody

Anti-PRKCA antibody (606-655 aa) (STJ95119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PKC alpha antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PRKCA antibody (658-672 aa) [R05-4E5] (STJA0013069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Anti-PRKCA antibody (Central region) [M237] (STJA0005531)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£394.50

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Immunofluorescence analysis of HeLa cells using PKC alpha Rabbit monoclonal antibody (STJ11105054) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKCA antibody [S5054RM] (STJ11105054)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£118.50 - £521.50

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Immunofluorescence analysis of Hela cell. 1, PKD1 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-PRKD1 antibody (171-220 aa) (STJ95136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using PKC mu Rabbit monoclonal antibody (STJ11101825) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PRKD1 antibody (200-300) [S5MR] (STJ11101825)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of PKC mu/PKD in C6, Hela lysates using PKD1 antibody.

Anti-PRKD1 antibody (230-245 aa) [R01-4B3] (STJA0011801)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£246.50

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Western blot analysis of PKC mu in K562, C6, Hela lysates using PKD1 antibody.

Anti-PRKD1 antibody (731-747 aa) [R05-4J5] (STJA0011800)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£246.50

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Western blot analysis of various lysates using PKC mu Rabbit polyclonal antibody (STJ118625) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PRKD1 antibody (800-900) (STJ118625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of NIH-3T3 cells using PKD2 Polyclonal Antibody

Anti-PRKD2 antibody (829-878 aa) (STJ95138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using PRKD3 antibody (STJ29164) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRKD3 antibody (1-100) (STJ29164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-PRKD3 antibody (STJ191980)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse lung using 3 Mu g PRKG2 antibody (STJ11100525). Western blot was performed from the immunoprecipitate using PRKG2 antibody (STJ11100525) at a dilution of 1:1000.

Anti-PRKG2 antibody (1-160) (STJ11100525)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using cGKII Polyclonal Antibody

Anti-PRKG2 antibody (391-440 aa) (STJ92257)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of A549 cells using cGKII Polyclonal Antibody diluted at 1：1000

Anti-PRKG2 antibody (92-141 aa) (STJ92256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PRMT4/CARM1 antibody [SRM14436] (STJ11109166)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of extracts of various cell lines, using PRODH antibody (STJ28399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRODH antibody (321-600) (STJ28399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PRODH antibody (STJA0007069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using PRPS1 Rabbit pAb (STJ11106806) at 1:1000 dilution. <br> Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br> Lysates/ proteins: 25 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Enhanced Kit <br> Exposure time: 40s.

Anti-PRPS1 antibody (250-322 aa) (STJ11106806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-PRPS1 antibody (STJA0006999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-PRPS1L1 antibody (STJA0009362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of various lysates using PRPS2 Rabbit polyclonal antibody (STJ114518) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRPS2 antibody (1-240) (STJ114518)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using PRPS2 Rabbit polyclonal antibody (STJ114519) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PRPS2 antibody (1-240) (STJ114519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-PRPS2 antibody (STJA0007311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using PRSS1 Rabbit pAb (STJ25163) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST.

Anti-PRSS1 antibody (24-247) (STJ25163)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various cells using Trypsin-1 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000

Anti-PRSS1 antibody (60-109 aa) (STJ96118)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using Trypsin-3 Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Anti-PRSS3 antibody (117-166 aa) (STJ96119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-PRXL2B antibody (STJA0008951)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from wild type (WT) and PSAP knockdown (KD) 293T cells using [KD Validated] PSAP Rabbit mAb (STJ11107290) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-PSAP antibody (275-524 aa) [SRM5379] (STJ11107290)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£143.50 - £618.50

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Immunofluorescence analysis of U2OS cells using PSAP Rabbit polyclonal antibody (STJ11104526) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PSAP antibody (275-524) (STJ11104526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U2OS cells using PSAP Rabbit polyclonal antibody (STJ25165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PSAP antibody (275-524) (STJ25165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 cells using Saposin Polyclonal Antibody

Anti-PSAP antibody (307-356 aa) (STJ95578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Anti-PSAP antibody [SRM14569] (STJ11109299)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£0.00

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Human prostate stained with anti-PSAP antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of benign prostate glands.

Anti-PSAP antibody [ZM162] (STJ180370)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

£161.50 - £347.50

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Western blot analysis of various lysates using PSAT1 Rabbit pAb (STJ116059) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PSAT1 antibody (1-370) (STJ116059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PSAT1 antibody (20-70) (STJA0006744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-PSMA antibody [SM5056] (STJA0005056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Western blot analysis of lysates from LNCaP cells, using PSMA/FOLH1 Rabbit mAb (STJ11103663) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-PSMA/FOLH1 antibody (1-106) [S3MR] (STJ11103663)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Anti-PSMA/FOLH1 antibody (50-150 aa) [SRM5319] (STJ11107223)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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