Metabolism | Research Area | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using HIGD1A Rabbit polyclonal antibody (STJ116792) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HIGD1A antibody (1-93) (STJ116792)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-HIGD1A antibody (STJA0007094)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-HIGD1C antibody (29-79 aa) (STJ196215)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of HILPDA in paraffin-embedded Mouse liver using HILPDA Rabbit polyclonal antibody (STJ11100178) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HILPDA antibody (1-63) (STJ11100178)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of 293T cells, using HK1 antibody (STJ11103807). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-HK1 antibody (20-300) (STJ11103807)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g HK1 antibody (STJ24019). Western blot was performed from the immunoprecipitate using HK1 antibody (STJ24019) at a dilution of 1:500.

Anti-HK1 antibody (21-219) (STJ24019)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of HXK I Polyclonal Antibody, using Hela, MCF7, SW480, 293T cell, 4°C over night, secondary antibody (cat： (NA was diluted at 1:10000, 37°C 1hour.

Anti-HK1 antibody (31-80 aa) (STJ93630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g [KO Validated] HK1 Rabbit monoclonal antibody (STJ11101239). Western blot was performed from the immunoprecipitate using HK1 (STJ11101239) at a dilution of 1:1000.

Anti-HK1 antibody (50-150) [S9MR] (STJ11101239)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis using HXK I monoclonal antibody against Jurkat (1) , HeLa (2) , HepG2 (3) and NIH/3T3 (4) cell lysate.

Anti-HK1 antibody [3A10] (STJ98151)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot detection of Hexokinase 1 in MCF7, Jurkat, CHO-K1, C2C12, C6, 3T3 and Hela cell lysates using Hexokinase 1 mouse mAb (1:1000 diluted). Predicted band size:102KDa. Observed band size:102KDa.

Anti-HK1 antibody [6B8-G11-E7] (STJ99104)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Anti-HK1 antibody [SRM14657] (STJ11109387)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using Hexokinase II Rabbit polyclonal antibody (STJ11103797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HK2 antibody (1-120) (STJ11103797)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of U2OS cells using Hexokinase II Rabbit polyclonal antibody (STJ24020) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HK2 antibody (1-120) (STJ24020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using HexokinaseII antibody (STJ11104798) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HK2 antibody (1-120) [S4798RM] (STJ11104798)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis using HXK II monoclonal antibody against PC12, HeLa, Jurkat cell lysate.

Anti-HK2 antibody (N-Term) (STJ98506)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Pig

£149.00 - £360.00

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Western blot detection of Hexokinase II in 3tT3, COS7 and C6 cell lysates using Hexokinase II mouse mAb (dilution 1:500).Predicted band size:102kDa.Observed band size:102kDa.

Anti-HK2 antibody [1E8-H3-F11] (STJ99120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Rat/Mouse/Monkey

£149.00 - £360.00

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Western blot analysis using HXK II monoclonal antibody against Jurkat (1) , HeLa (2) and HEK293 (3) cell lysate.

Anti-HK2 antibody [3D3] (STJ98152)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis of various lysates using HK3 Rabbit pAb (STJ110726) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-HK3 antibody (1-270) (STJ110726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Jurkat cells, treated with insulin 0.01U/ml 15', using Hexokinase-3 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-HK3 antibody (811-860 aa) (STJ93631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using HKDC1 Rabbit polyclonal antibody (STJ119000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HKDC1 antibody (150-250) (STJ119000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-HKDC1 antibody (STJA0008022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-HLCS antibody (STJA0009414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using HMBS antibody (STJ24034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HMBS antibody (1-361) (STJ24034)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using HMBS Rabbit monoclonal antibody (STJ11101395) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-HMBS antibody (250-350) [S5MR] (STJ11101395)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Anti-HMBS antibody (STJA0009583)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using HMGCL Rabbit pAb (STJ110355) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 15s.

Anti-HMGCL antibody (1-325) (STJ110355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HMGCL antibody (C-Term) (STJA0010324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HMGCLL1 antibody (STJA0004619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-HMGCLL1 antibody (STJA0004620)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£140.50 - £415.50

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Anti-HMGCLL1 antibody (STJA0008870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells using HMGCS1 Rabbit polyclonal antibody (STJ24047) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMGCS1 antibody (241-520) (STJ24047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HMGCS1 antibody (STJ190905)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates, using HMGCS2 rabbit polyclonal antibody (STJ116457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMGCS2 antibody (269-508) (STJ116457)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-HMGCS2 antibody (STJA0009800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-HMGCS2 antibody [SRM14665] (STJ11109395)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemistry analysis of paraffin-embedded human esophageal using CD168/RHAMM Rabbit monoclonal antibody (STJ11101383) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HMMR antibody (1-100) [S3MR] (STJ11101383)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Immunofluorescence analysis of U2OS cells using [KO Validated] CD168/RHAMM Rabbit polyclonal antibody (STJ114319). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HMMR antibody (1-300) (STJ114319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£126.50 - £618.50

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Western blot analysis of 293T lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-HMMR antibody (280-340 aa) (STJ98724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of Heme Oxygenase 1 (HO-1/HMOX1) in paraffin-embedded Rat spleen using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ113706) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HMOX1 antibody (1-265) (STJ113706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of Raw264. 7 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (STJ11101600) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HMOX1 antibody (1-265) [S0MR] (STJ11101600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of various lysates, using HemeOxygenase1 (HO-1/HMOX1) antibody (STJ11104601) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMOX1 antibody (1-265) [S4601RM] (STJ11104601)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £554.50

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Immunofluorescence analysis of HepG2 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ116419) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMOX1 antibody (1-288) (STJ116419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 3T3 Cell lysate using HO-1 mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HMOX1 antibody [Mix] (STJ196663)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Immunohistochemistry analysis of Heme Oxygenase 1 (HO-1/HMOX1) in paraffin-embedded mouse liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit monoclonal antibody (STJ11105129) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HMOX1 antibody [S5129RM] (STJ11105129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Western blot analysis of various lysates using HMOX2 Rabbit polyclonal antibody (STJ24054) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMOX2 antibody (1-270) (STJ24054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HMOX2 antibody (STJ192960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells using 1 Mu g HNMT antibody (STJ115460). Western blot was performed from the immunoprecipitate using HNMT antibody (STJ115460) at a dilution of 1:1000.

Anti-HNMT antibody (1-292) (STJ115460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of HT-29 cells using 1 Mu g HNMT antibody (STJ24061). Western blot was performed from the immunoprecipitate using HNMT antibody (STJ24061) at a dilution of 1:1000. ____Detection: ECL Enhanced Kit. _Exposure time: 10s.

Anti-HNMT antibody (1-292) (STJ24061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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