Metabolism Of Proteins | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Alpha-Tubulin rabbit monoclonal antibody (STJ11102329) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TUBA4A antibody (349-448) [S9MR] (STJ11102329)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£169.50 - £314.50

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Immunocytochemistry analysis of alpha Tubulin (green) in hela using alpha Tubulin antibody, and DAPI (blue).

Anti-TUBA4A antibody (400-448 aa) [R02-2H3] (STJA0010943)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat/Hamster

£168.50 - £727.50

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Anti TUBA4A Goat Polyclonal antibody immunofluorescence mouse STJ140051

Anti-TUBA4A antibody (400aa C-Term) (STJ140051)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £686.50

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Anti TUBA4A Goat Polyclonal antibody western blot STJ140087

Anti-TUBA4A antibody (80aa N-Term) (STJ140087)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian

£94.50 - £440.50

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Anti-TUBA8 antibody (STJ191896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TUBB1 antibody (257-307 aa) (STJ192010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of Zebrafish skeletal muscle, diluted at 1:5000.

Anti-TUBB1 antibody (STJ97021)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Zebrafish

£49.00 - £261.00

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Western blot analysis using Tubulin Beta monoclonal antibody against A431, HeLa, Jurkat cell lysate.

Anti-TUBB1 antibody (STJ98566)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat/Cow/Rabbit

£149.00 - £360.00

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Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, Beta I tubulin monoclonal antibody (3F7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-TUBB1 antibody [3F7] (STJ96939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Kidney tissue, diluted at 1:3000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-TUBB2A antibody (80-130 aa) (STJ197320)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human-colon tissue. 1, Beta II tubulin monoclonal antibody (Mix) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-TUBB2A antibody [Mix] (STJ97053)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) Mouse Brain, 3) Rat Brain tissue, diluted at 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-TUBB3 antibody (134-184 aa) (STJ197319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Beta III-Tubulin/Beta 3-Tubulin antibody (STJ11101457) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-TUBB3 antibody (351-450) [S7MR] (STJ11101457)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various lysates, using Beta III-Tubulin Rabbit polyclonal antibody (STJ119293) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-TUBB3 antibody (40-260) (STJ119293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded mouse brain using [KO Validated] Beta III-Tubulin Mouse monoclonal antibody (STJ11100102) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-TUBB3 antibody (40-260) [S2MM] (STJ11100102)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Beta III-Tubulin/Beta 3-Tubulin antibody (STJ119294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TUBB3 antibody (40-450) (STJ119294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of human-uterus tissue. 1, Tubulin Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-TUBB3 antibody (401-450 aa) (STJ96145)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig/Cow/Fish

£49.00 - £261.00

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Anti-TUBB3 antibody (C-Term) (STJA0005630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£319.50

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Anti-TUBB3 antibody (C-Term) (STJA0005804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£488.50

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Anti-TUBB3 antibody (central Region) (STJA0005629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Anti-TUBB3 antibody (STJ197318)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Zebrafish skeletal muscle, (Zebrafish Specific) diluted at 1:5000.

Anti-TUBB3 antibody (STJ97016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Zebrafish/T.fulvidraco/Tetraodontidae

£49.00 - £261.00

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Anti-TUBB3 antibody (STJ97017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Zebrafish

£49.00 - £261.00

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Anti-TUBB3 antibody (STJA0005632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 350} (STJA0006205)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 405} (STJA0006206)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {AbFluor™ 488} (STJA0006207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 555} (STJA0006208)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TUBB3 antibody [5G3] {AbFluor™ 594} (STJA0006209)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {AbFluor™ 647} (STJA0006210)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {AbFluor™ 680} (STJA0006211)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {Biotin} (STJA0006212)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {Cy3} (STJA0006213)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {Cy5} (STJA0006214)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {FITC} (STJA0006215)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-TUBB3 antibody [5G3] {HRP} (STJA0006216)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Sheep/Insect/Yeast

£149.00 - £248.00

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Immunofluorescence analysis of Hela cell. 1, ERK 1/2 (phospho Tyr222/205) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-tubulin monoclonal antibody (M7) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-TUBB3 antibody [M7] (STJ97037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TUBB4A antibody (218-268 aa) (STJ192957)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunocytochemistry analysis of beta IV Tubulin (green) in Hela using beta IV Tubulin antibody, and DAPI (blue)

Anti-TUBB4A antibody (430-444 aa) [R06-6A5] (STJA0011034)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunofluorescence analysis of U2OS cells using TUBB8 antibody (STJ11102746) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TUBB8 antibody (344-444 aa) (STJ11102746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of various lysates using TUBB8 Rabbit pAb (STJ11105740) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-TUBB8 antibody (350-444) (STJ11105740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-Tubulin Alpha antibody [TU-02] (STJ16100478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC

Reactivity :

Human/Mouse/Pig/PlantS./Rat

£331.50 - £1,476.50

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Immunofluorescence analysis of U-2 OS cells using Tubulin beta-1 chain Rabbit monoclonal antibody (STJ11103147) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Tubulin beta-1 chain antibody (352-451) [S7MR] (STJ11103147)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Cow/Dog/Monkey

£118.50 - £521.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g TUFM antibody (STJ28506). Western blot was performed from the immunoprecipitate using TUFM antibody (STJ28506) at a dilution of 1:1000.

Anti-TUFM antibody (186-455) (STJ28506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using TUFM Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TUFM antibody (301-350 aa) (STJ92844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-TUFM antibody (325-375) (STJ506047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Anti-TUFM antibody (325-375) {Biotin} (STJ506048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-TUFM antibody (325-375) {FITC} (STJ506049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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