Immune System | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using CD152/CTLA-4 antibody (STJ115900) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTLA4 antibody (49-223) (STJ115900)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain using 3 Mu g Beta-Catenin antibody (STJ11103800). Western blot was performed from the immunoprecipitate using Beta-Catenin antibody (STJ11103800) at a dilution of 1:1000.

Anti-CTNNB1 antibody (1-100) (STJ11103800)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £642.50

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Immunofluorescence analysis of U2OS cells using Beta-Catenin antibody (STJ113475) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CTNNB1 antibody (1-100) (STJ113475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £642.50

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Western blot analysis of extracts of HeLa cells, using Beta-Catenin antibody (STJ11101750) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CTNNB1 antibody (1-100) [S0MR] (STJ11101750)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various cells using Catenin-Beta Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (3-52 aa) (STJ92041)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/CoIP/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry of rat tissue with Anti-CTNNB1 primary antibody (STJ500624) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CTNNB1 antibody (50-100) (STJ500624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CTNNB1 antibody (50-100) {Biotin} (STJ500626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CTNNB1 antibody (50-100) {FITC} (STJ500627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of various cells using Catenin-Beta 1 Polyclonal Antibody diluted at 1：1000

Anti-CTNNB1 antibody (626-675 aa) (STJ92042)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts from normal (control) and Beta-Catenin knockout (KO) 293T cells, using Beta-Catenin antibody (STJ113088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CTNNB1 antibody (650-750) (STJ113088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Beta-Catenin antibody (STJ113822). Western blot was performed from the immunoprecipitate using Beta-Catenin antibody (STJ113822) at a dilution of 1:1000.

Anti-CTNNB1 antibody (667-781) (STJ113822)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Beta-catenin antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 94 kDa

Anti-CTNNB1 antibody (700-781 aa) [ABT-Catenin] (STJ197008)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunohistochemistry of mouse lung using anti-CTNNB1 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CTNNB1 antibody at 1:500:DAB detection.

Anti-CTNNB1 antibody (731aa C-Term) (STJ140021)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot with Anti-CTNNB1 Antibody (STJ500625) with peptide on at 1:500 dilution in buffer.

Anti-CTNNB1 antibody (740-790) (STJ500625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CTNNB1 antibody (740-790) {Biotin} (STJ500628)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CTNNB1 antibody (740-790) {FITC} (STJ500629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CTNNB1 antibody (C-Term) [M118] (STJA0005301)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human/Mouse/Rat

£390.50

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Anti-CTNNB1 antibody (N-Term) (STJA0005302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of 1) 3T3, 2) Rat Liver, diluted at 1:2,000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-CTNNB1 antibody (STJ197317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse/Rat/Rabbit

£48.00 - £252.00

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Anti-CTNNB1 antibody (STJA0009925)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using Catenin-Beta monoclonal antibody against CTNNB1-hIgGFc transfected HEK293 cell lysate.

Anti-CTNNB1 antibody [4D5] (STJ97896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£144.00 - £348.00

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Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, Catenin-Beta monoclonal antibody (4F2) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CTNNB1 antibody [4F2] (STJ97420)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat/Zebrafish

£48.00 - £252.00

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Anti-CTNNB1 nanobody [SAA1188] (STJN000206)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/SPR/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-CTNNB1 nanobody [SAA1197] (STJN000205)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/IF/IHC/IP/SPR/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-CTNNB1 nanobody [SAA1410] (STJN000526)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

£378.50 - £1,632.50

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Western blot analysis of extracts of various cell lines, using CTSA antibody (STJ27454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CTSA antibody (210-450) (STJ27454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using Cathepsin B Polyclonal Antibody

Anti-CTSB antibody (168-217 aa) (STJ92044)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using Cathepsin B Rabbit polyclonal antibody (STJ23278) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CTSB antibody (18-339) (STJ23278)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using Cathepsin B Rabbit monoclonal antibody (STJ11101543) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CTSB antibody (27-156) [S3MR] (STJ11101543)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£129.50 - £574.50

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Confocal imaging of Hep G2 cells using Cathepsin B Rabbit monoclonal antibody (STJ11104983, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-CTSB antibody (8-339) [S4983RM] (STJ11104983)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CTSB antibody [2A2] (STJA0021587)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£341.50 - £1,447.50

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Immunofluorescence analysis of L929 cells using CTSC Rabbit pAb (STJ110701) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CTSC antibody (25-134) (STJ110701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded human tonsil using Cathepsin D Rabbit polyclonal antibody (STJ115256) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CTSD antibody (21-249) (STJ115256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CTSD antibody at 1:500 dilution; endogenous CTSD (100 µg per lane) and transfected 293FT cell lysate (at 30 µg per lane) ; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CTSD antibody (275aa C-Term) (STJ140018)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/IEM

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Western blot analysis of various cells using Cathepsin D Polyclonal Antibody

Anti-CTSD antibody (296-345 aa) (STJ92046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of Cathepsin D in paraffin-embedded mouse kidney tissue using Cathepsin D Rabbit monoclonal antibody (STJ11101762) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CTSD antibody (313-412) [S2MR] (STJ11101762)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Cathepsin D antibody (STJ23280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-CTSD antibody (65-412) (STJ23280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of HeLa cells using Cathepsin D Polyclonal Antibody

Anti-CTSD antibody (Internal) (STJ92045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-CTSD/Cathepsin D antibody [SAA1766] (STJA0020194)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Cathepsin E (CTSE) antibody (STJ23281) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CTSE antibody (132-244) (STJ23281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Cathepsin E (CTSE) antibody (STJ11104701) at1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CTSE antibody (132-244) [S4701RM] (STJ11104701)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various cells using Cathepsin E Polyclonal Antibody

Anti-CTSE antibody (243-292 aa) (STJ92047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of CTSF in paraffin-embedded human breast cancer using CTSF Rabbit polyclonal antibody (STJ112496) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CTSF antibody (270-484) (STJ112496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various lysates, using Cathepsin G Rabbit monoclonal antibody (STJ11104681) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CTSG antibody (156-255) [S4681RM] (STJ11104681)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Anti-CTSG antibody (21-255) (STJ23282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of THP-1 cells using Cathepsin G Rabbit polyclonal antibody (STJ27603) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CTSG antibody (21-255) (STJ27603)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of COLO205 cells using Cathepsin G Polyclonal Antibody

Anti-CTSG antibody (67-116 aa) (STJ92048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of Cathepsin G in paraffin-embedded mouse spleen using Cathepsin G Rabbit polyclonal antibody (STJ115138) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CTSG antibody (75-190) (STJ115138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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