Immune System | Research Area | St John's Laboratory

Immunofluorescence analysis of NIH/3T3 cells using COL1A1 Rabbit polyclonal antibody (STJ11105651) at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A1 antibody (1359-1464) (STJ11105651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of paraffin-embedded human colon using Human Pro-Collagen I alpha Rabbit polyclonal antibody (STJ11105505) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-COL1A1 antibody (26-161) (STJ11105505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-COL1A1 antibody (STJ98915)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of human-stomach tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-COL1A2 antibody (1-50 aa) (STJ92383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ119111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-COL1A2 antibody (1161-1260) (STJ119111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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PC-12 whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-Collagen alpha-1 (I) chain (ABT162) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: PC-12Predicted band size: 139, 115kDaObserved band size: 125, 105kDa

Anti-COL1A2 antibody (1200-1464 aa) [ABT162] (STJ197220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of various cells using COL1A2 Polyclonal Antibody diluted at 1：1000

Anti-COL1A2 antibody (471-520 aa) (STJ92384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ28351) at dilution of 1:25. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A2 antibody (501-600) (STJ28351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-COL1A2 antibody (STJ99633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 1) k562, 2) COLO205, 3) MCF-7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-COL2A1 antibody (101-150 aa) (STJ92387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Human cartilage tissue was stained with Anti-Collagen Type II (ABT501) Antibody

Anti-COL2A1 antibody (1200-1300 aa) [ABT501] (STJ197134)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunofluorescence analysis of paraffin-embedded rat trachea using COL2A1 Rabbit polyclonal antibody (STJ11103919) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL2A1 antibody (1200-1300) (STJ11103919)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of HepG2 cells using COL2A1 Rabbit polyclonal antibody (STJ23191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL2A1 antibody (1220-1282) (STJ23191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-COL2A1 antibody (STJ98944)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Human colon tissue was stained with Anti-Collagen Type III (ABT-CIII) Antibody

Anti-COL3A1 antibody (100-200 aa) [ABT-CIII] (STJ196885)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£174.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-COL3A1 antibody (100-200 aa) [PT0118] (STJ196955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 Rabbit polyclonal antibody (STJ23192) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL3A1 antibody (1201-1301) (STJ23192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunocytochemistry analysis of Collagen III (green) in Hela using Collagen III antibody, and DAPI (blue)

Anti-COL3A1 antibody (201-400 aa) [R06-4E1] (STJA0012676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Immunofluorescence analysis of HeLa cells, using Collagen III Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL3A1 antibody (81-130 aa) (STJ92388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-COL3A1 antibody [ABT192R] (STJ197075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-COL3A1 antibody [Q76] (STJ97025)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human prostate using COLEC11 antibody (STJ26983) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-COLEC11 antibody (26-271) (STJ26983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-COLEC11 antibody (STJA0007188)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using COLEC12 antibody (STJ112454) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-COLEC12 antibody (60-270) (STJ112454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Anti-COLEC12 antibody (STJ192053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot of rat lung lysate showing specific immunolabeling of the ~180 kDa collagen 1.

Anti-Collagen 1, alpha 1 propeptide antibody (STJA0003599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/Feline/Finch/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus

£454.50

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Immunostaining of formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (STJA0003600, 1:100, red) that has formed fibrils in the extracellular matrix. The blue is staining nuclear DNA.

Anti-Collagen 1, alpha 1 telopeptide antibody (STJA0003600)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat/Sheep/Bovine/Canine/Chicken/Feline/Finch/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Vole/Xenopus

£454.50

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Anti-Collagen I antibody [SM0490] (STJA0010490)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Collagen II antibody [SM5168] (STJA0005168)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Immunoprecipitation analysis of 300 Mu g extracts from HepG2 cells using 3 Mu g Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 Rabbit monoclonal antibody (STJ11102715) at a dilution of 1:1000.

Anti-Collagen III alpha 1/COL3A1 antibody (150-400) [S5MR] (STJ11102715)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Collagen III antibody [SM0439] (STJA0010439)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of lysates from MCF-7 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-COMMD3 antibody (24-74 aa) (STJ195817)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-COMMD9 antibody (STJA0004840)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Anti-COMMD9 antibody (STJA0004841)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£140.50 - £415.50

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Confocal imaging of paraffin-embedded Human liver cancer tissue using Complement C4A Rabbit monoclonal antibody (STJ11103522, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Complement C4A antibody (940-1237) [S2MR] (STJ11103522)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of lysates from BxPC-3 cells, using Complement factor H Rabbit mAb (STJ11103633) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Complement factor H antibody (100-200) [S3MR] (STJ11103633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of HeLa cells using COPB1 Rabbit polyclonal antibody (STJ112510) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COPB1 antibody (460-740) (STJ112510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-COPB1 antibody (865-915 aa) (STJ192922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse lung using COTL1 Rabbit polyclonal antibody (STJ23203) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-COTL1 antibody (1-142) (STJ23203)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-COTL1 antibody (29-79 aa) (STJ195029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of COX2/PTGS2 in paraffin-embedded rat colon tissue using COX2/PTGS2 Rabbit monoclonal antibody (STJ11103523) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-COX2/PTGS2 antibody (505-604) [S3MR] (STJ11103523)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-CPB2 antibody (115-365 aa) (STJ11106088)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of various lysates using CPB2 Rabbit pAb (STJ27601) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-CPB2 antibody (115-365) (STJ27601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of lysates from HepG2 cells, using CPB2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-CPB2 antibody (321-370 aa) (STJ95898)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CPN1 Rabbit pAb (STJ110197) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-CPN1 antibody (209-458) (STJ110197)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using CPN cat Polyclonal Antibody

Anti-CPN1 antibody (409-458 aa) (STJ92454)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CPN2 antibody (C-Term) (STJ92455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various lysates using CPNE1 Rabbit polyclonal antibody (STJ118194) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CPNE1 antibody (120-220) (STJ118194)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

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