Immune System | Research Area | St John's Laboratory

Western blot analysis of lysates from Raji cells, using CD79B Rabbit monoclonal antibody (STJ11105118) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-CD79B antibody [S5118RM] (STJ11105118)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-CD8 alpha antibody (1-50) (STJ505971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-CD8 alpha antibody (1-50) {Biotin} (STJ505972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-CD8 alpha antibody (1-50) {FITC} (STJ505973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Immunohistochemistry analysis of CD8A. Sample: Human colon cancer, 4% PFA 12-24h. Antigen retrieval: Citrate buffer, pressure cooker 2min. Blocking buffer: 3% BSA in PBS, RT, 30min. Primary antibody: 1:3000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:200 RT 1h.

Anti-CD8 alpha antibody [SM4941] (STJA0004941)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Human tonsil stained with anti-CD8 antibody using peroxidase-conjugate and DAB chromogen. Not the membrane staining of perifollicular T-cells.

Anti-CD8 antibody [ZR286] (STJ180498)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£183.50 - £705.50

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Anti-CD8 beta antibody (70-120) (STJ505974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CD8 beta antibody (70-120) {Biotin} (STJ505975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CD8 beta antibody (70-120) {FITC} (STJ505976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry analysis of CD80 in paraffin-embedded human spleen using CD80 Rabbit polyclonal antibody (STJ118479) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD80 antibody (1-100) (STJ118479)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-CD80 antibody (100-160 aa) (STJ98977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human Tonsil tissues with AEC staining using CD80 monoclonal antibody.

Anti-CD80 antibody [2A2] (STJ97938)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Daudi (human B Burkitt's lymphoma cell line) cells were stained with Anti-CD80 antibody (STJA0001114) (filled gray histogram).Unstained Daudi cells (empty black histogram) were used as control.

Anti-CD80 antibody [2D10] {APC} (STJA0001114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£124.50 - £298.50

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Anti-CD80 antibody [2D10] {PE/Cyanine7} (STJA0002584)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£149.50 - £331.50

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Anti-CD80 antibody [2D10] {PE} (STJA0002239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£125.50 - £261.50

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Anti-CD80 antibody [C80-67] (STJ16102644)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Anti-CD80 antibody [C80-99] (STJ16101125)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£287.50 - £1,324.50

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Anti-CD80 antibody [mAb104] (STJ16102075)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FACS

Reactivity :

Human

£415.50

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Anti-CD80 antibody [MEM-233] (STJ16100188)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IP

Reactivity :

Human

£431.50

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Anti-CD80 antibody [MEM-233] {FITC} (STJ16100189)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£537.50

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Western blot analysis of various lysates, using CD80 Rabbit monoclonal antibody antibody (STJ11105144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CD80 antibody [S5144RM] (STJ11105144)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates, using CD81 Rabbit monoclonal antibody (STJ11102173) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CD81 antibody (100-200) [S3MR] (STJ11102173)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from HT-29, NIH/3T3, and HepG2 cells, primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat： (NA) was diluted at 1:10000, 37°C 1hour.

Anti-CD81 antibody (111-160 aa) (STJ96759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-CD81 antibody (114-199 aa) [SRM] (STJ11106904)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of THP-1 and U-937 cells using CD81 Rabbit polyclonal antibody (STJ111247) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-CD81 antibody (114-199) (STJ111247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of CD81 in C2C12, 3T3, MCF-7 lysates using CD81 antibody.

Anti-CD81 antibody (34-63+113-201 aa) [R02-3D6] (STJA0013040)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse

£264.50

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Anti-CD81 antibody [1.3.3.22] (STJ16100041)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£287.50 - £1,324.50

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Anti-CD81 antibody [1.3.3.22] {AF488} (STJA0002671)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£149.50 - £352.50

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Anti-CD81 antibody [1.3.3.22] {APC} (STJA0002670)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£133.50 - £298.50

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Anti-CD81 antibody [1.3.3.22] {EF700} (STJA0016360)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£156.50 - £352.50

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Anti-CD81 antibody [1.3.3.22] {FITC} (STJA0002668)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£121.50 - £281.50

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Anti-CD81 antibody [1.3.3.22] {PE/TR} (STJA0004237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£149.50 - £352.50

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Anti-CD81 antibody [1.3.3.22] {PE} (STJA0002669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£124.50 - £298.50

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Anti-CD81 antibody [1D6] (STJ16100575)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IP/FC/IF

Reactivity :

Human/Sheep/Chimpanzee/Goat

£741.50

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Western blot analysis of extracts of various cell lines, using CD86 antibody (STJ11101564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-CD86 antibody (1-100) [S4MR] (STJ11101564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of CD86 in paraffin-embedded mouse spleen using CD86 Rabbit polyclonal antibody (STJ113561) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CD86 antibody (24-247) (STJ113561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunofluorescence analysis of Raji cells using CD86 Rabbit polyclonal antibody (STJ23014) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD86 antibody (25-133) (STJ23014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunoprecipitation analysis of 200 Mu g extracts of Jurkat cells using 3 Mu g CD86 antibody (STJ119185). Western blot was performed from the immunoprecipitate using CD86 antibody (STJ119185) at a dilution of 1:1000.

Anti-CD86 antibody (25-329) (STJ119185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot with Anti-CD86 Antibody (STJ500486) with peptide on at 1:500 dilution in buffer.

Anti-CD86 antibody (250-329) (STJ500486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

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Anti-CD86 antibody (250-329) {Biotin} (STJ500487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-CD86 antibody (250-329) {FITC} (STJ500488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-CD86 antibody (STJA0010129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis using CD86 monoclonal antibody against L1210 (1) and MOLT-4 (2) cell lysate.

Anti-CD86 antibody [1B3] (STJ97939)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-CD86 antibody [BU63] (STJ16100509)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IP/FC/IHC-F

Reactivity :

Human

£741.50

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Anti-CD86 antibody [BU63] (STJA0001270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/Block/IP

Reactivity :

Human

£123.50 - £187.50

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Human peripheral blood monocytes were stained with Anti-CD86 antibody (STJA0000179) (filled gray histogram). Unstained monocytes (empty black histogram) were used as control.

Anti-CD86 antibody [BU63] {AF488} (STJA0000179)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£135.50 - £319.50

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Human peripheral blood monocytes were stained with Anti-Human CD86 Monoclonal Antibody (AF647 Conjugated) (filled gray histogram). Unstained monocytes (empty black histogram) were used as control.

Anti-CD86 antibody [BU63] {AF647} (STJA0000180)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£135.50 - £319.50

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Human peripheral blood monocytes were stained with Anti-CD86 antibody (STJA0000176) (filled gray histogram). Unstained monocytes (empty black histogram) were used as control.

Anti-CD86 antibody [BU63] {APC} (STJA0000176)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£142.50 - £357.50

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