Immune System | Research Area | St John's Laboratory

Anti-Phospho-CFL1-Ser-3 antibody (STJA0005346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Western blot analysis of NIH-3T3 cells using Phospho-Cofilin (S3) Polyclonal Antibody

Anti-Phospho-CFL1-Ser3 antibody (1-50 aa) (STJ90230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-CFL1-Y139 Rabbit pAb (STJ11105777) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Bufferr (pH 6. 0) prior to immunohistochemistry staining.

Anti-Phospho-CFL1-Y139 antibody (STJ11105777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-CGAS-S291 antibody (STJ11101078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-CGAS-Y215 Rabbit pAb (STJ11107679) at 1:1000 dilution. NIH/3T3 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CGAS-Y215 antibody (STJ11107679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-IKK Alpha-S180 Rabbit pAb (STJ110846) at 1:1000 dilution. HeLa cells were treated by both TNF-Alpha (20ng/mL) and Calyculin A (100nM) for 10 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CHUK-S180 antibody (STJ110846)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-CHUK-T23 antibody (STJ11101104)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-IKK Alpha (T23) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CHUK-Thr23 antibody (15-64 aa) (STJ90300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 293T cells, using Phospho-c-Jun-S63 Rabbit pAb (STJ11103730) at 1:1000 dilution. 293T cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-cJun-S63 antibody (STJ11103730)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T91 antibody (STJ11103749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (STJ11103751) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37 °C for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T93 antibody (STJ11103751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Anti-Phospho-Cofilin antibody (1-50) (STJ506121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-Phospho-Cofilin antibody (1-50) {Biotin} (STJ506122)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-Cofilin antibody (1-50) {FITC} (STJ506123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

£584.50

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Anti-Phospho-CREB1-S119 antibody [P5G10] (STJA0019174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human/Mouse/Rat/Bovine

£378.50 - £1,632.50

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Western blot analysis of lysates from 293T cells, using Phospho-CREB1-S129 Rabbit pAb (STJ11101038) at 1:500 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CREB1-S129 antibody (STJ11101038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-CREB1-S133 Rabbit pAb (STJ227724). HeLa cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-CREB1-S133 antibody (STJ22076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CREB1-S133 Rabbit pAb (STJ22079) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes or treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-CREB1-S133 antibody (STJ22079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded rat colon using Phospho-CREB1-S133 Rabbit monoclonal antibody (STJ11105877) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-CREB1-S133 antibody [S5877RM] (STJ11105877)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-CREB1-S133 antibody [SAA0304] (STJA0019173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-CREB1-S142 antibody (STJ11105778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-CREB1-Ser-133 antibody [M415] (STJA0005351)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£319.50

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Anti-Phospho-CREB1-Ser-271 antibody (STJA0005352)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£365.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using CREB (Phospho-Ser133) antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-Phospho-CREB1-Ser133 antibody (26-140 aa) [R07-3C4] (STJA0011169)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Rat

£246.50

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Anti-Phospho-CRK-Tyr-221 antibody (STJA0005354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-CRK-Tyr-251 antibody (STJA0005355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of various cells using Phospho-Crk II (Y221) Polyclonal Antibody

Anti-Phospho-CRK-Tyr221 antibody (187-236 aa) (STJ90687)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from K-562 cells, using Phospho-CRK/CRKII-Y221 Rabbit pAb (STJ117920) at 1:1000 dilution. K-562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody (STJ117920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (STJ11102643) at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-CRK-Y221 antibody [S3MR] (STJ11102643)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Phospho-CRKL-Tyr-207 antibody (STJA0005357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of various cells using Phospho-Crk-L (Y207) Polyclonal Antibody

Anti-Phospho-CRKL-Tyr207 antibody (173-222 aa) (STJ91029)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells using Phospho-CRKL-Y207 Rabbit pAb (STJ11107726) at1:500 dilution. NIH/3T3 cells were treated by Î»-PP mixed solution (1ul) at 30C for 30 minutes. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 180s.

Anti-Phospho-CRKL-Y207 antibody (STJ11107726)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£129.50 - £642.50

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Western blot analysis of lysates from K-562 cells, using Phospho-CRKL-Y207 Rabbit pAb (STJ117922) at 1:1000 dilution or CRKL antibody (STJ230245). K-562 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-Phospho-CRKL-Y207 antibody (STJ117922)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CSF1R-Tyr546 antibody (STJ196355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of HepG2 cells using Phospho-c-Fms (Y561) Polyclonal Antibody

Anti-Phospho-CSF1R-Tyr561 antibody (531-580 aa) (STJ90894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-CSF1R-Tyr699 antibody (STJ90664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-CSF1R-Tyr708 antibody (STJ90665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30', using M-CSF Receptor (Phospho-Tyr723) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CSF1R-Tyr723 antibody (691-740 aa) (STJ90853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 using p-c-Fms (Y809) antibody.

Anti-Phospho-CSF1R-Tyr809 antibody (781-830 aa) (STJ90468)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using CSFR (Phospho-Tyr809) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CSF1R-Tyr809 antibody (781-830 aa) (STJ91144)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-CSF1R-Tyr921 antibody (STJA0010045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-CSF1R-Tyr923 antibody (STJ196356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-CSF1R-Y699 antibody (STJ29373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from K-562 cells, using Phospho-CD115/CSF-1R-Y708 Rabbit pAb (STJ29375) at 1:1000 dilution. K-562 cells were treated by G-CSF (25 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CSF1R-Y708 antibody (STJ29375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from wild type (WT) and 293T transfected with Phospho-CD115/CSF-1R-Y723, using Phospho-CD115/CSF-1R-Y723 (STJ11101113) at 1:500 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CSF1R-Y723 antibody (STJ11101113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CSF2RB-Tyr593 antibody (559-608 aa) (STJ90799)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of VEC cells using Phospho-Csk (S364) Polyclonal Antibody

Anti-Phospho-CSK-Ser364 antibody (330-379 aa) (STJ90456)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 MCF-7 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-CSK-Tyr304 antibody (STJA0010069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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