Haemostasis | Research Area | St John's Laboratory

Western blot analysis of various cells using Phospho-PKC Delta (S645) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCD-Ser645 antibody (612-661 aa) (STJ91011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

€61.25 - €326.25

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Western blot analysis of lysates from U-937 cells, using Phospho-PKC delta-T505 Rabbit pAb (STJ116396) at 1:2000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PRKCD-T505 antibody (STJ116396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Western blot analysis of 3T3 cells using Phospho-PKC Delta (T507) Polyclonal Antibody

Anti-Phospho-PRKCD-Thr507 antibody (471-520 aa) (STJ90388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from HepG2 cells, COLO205 cells and HUVEC cells, using PKC delta (Phospho-Tyr313) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr313 antibody (279-328 aa) (STJ90864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PKC delta (Phospho-Tyr52) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr52 antibody (18-67 aa) (STJ91068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using PKCD (Phospho-Tyr64) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCD-Tyr64 antibody (30-79 aa) (STJ90593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from U937 and Raw264. 7 cells, using Phospho-PKC delta-Y311 Rabbit pAb (STJ117870) at 1:1000 dilution. U-937 cells were treated by PMA/TPA (200nM) for 30 minutes. RAW 264. 7 cells were treated by PMA (200 nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PRKCD-Y311 antibody (STJ117870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€158.13 - €773.13

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Western blot analysis of lysates from HeLa cells treated with PMA 125ng/ml 30', using PKC epsilon (Phospho-Ser729) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCE-Ser729 antibody (688-737 aa) (STJ90500)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from Jurkat cells, using Phospho-PRKCQ-S676 Rabbit pAb (STJ11105774) at 1:1000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PRKCQ-S676 antibody (STJ11105774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC thet (Phospho-Ser676) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCQ-Ser676 antibody (643-692 aa) (STJ90391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 15', using PKC thet (Phospho-Ser695) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PRKCQ-Ser695 antibody (657-706 aa) (STJ90879)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from Jurkat and HeLa cells, using Phospho-PRKCQ-T538 Rabbit pAb (STJ117896) at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes. HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-PRKCQ-T538 antibody (STJ117896)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKCQ-Thr538 antibody (504-553 aa) (STJ90865)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using Phospho-PKC zeta-T560 Rabbit mAb (STJ11102642) at 1:1000 dilution. Both 293T cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-PRKCZ-T560 antibody [S2MR] (STJ11102642)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€148.13 - €651.88

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Western blot analysis of various cells using Phospho-PKC Zeta (T410) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PRKCZ-Thr410 antibody (376-425 aa) (STJ90389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HELA 293T cells using Phospho-PKC Zeta (T560) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-PRKCZ-Thr560 antibody (526-575 aa) (STJ90390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PRKG2-Ser126 antibody (92-141 aa) (STJ91216)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-PTK2-Ser722 antibody (STJA0009853)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-PTK2-Ser732 antibody (STJ193214)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of HELA KB 293T 3T3 lysis using Phospho-FAK (S843) antibody. Antibody was diluted at 1:1000

Anti-Phospho-PTK2-Ser843 antibody (809-858 aa) (STJ90571)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of HepG2 cells, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Ser910 antibody (876-925 aa) (STJ91306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-PTK2-Tyr-397 antibody [M121] (STJA0005417)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Rabbit

€456.88

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Western blot analysis of lysates from Jurkat cells treated with Ca2+ 40nM 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Tyr397 antibody (363-412 aa) (STJ90899)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 293 cells using Phospho-FAK (Y397) Polyclonal Antibody

Anti-Phospho-PTK2-Tyr397 antibody (364-413 aa) (STJ90675)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-FAK (Y407) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTK2-Tyr407 antibody (373-422 aa) (STJ90809)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot analysis of 3T3 cells using Phospho-FAK (Y576) Polyclonal Antibody

Anti-Phospho-PTK2-Tyr576 antibody (542-591 aa) (STJ90789)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FAK (Phospho-Tyr577) Antibody

Anti-Phospho-PTK2-Tyr577 antibody (543-592 aa) (STJ91185)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-FAK (Y861) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTK2-Tyr861 antibody (828-877 aa) (STJ90268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot analysis of lysates from Jurkat and HepG2 cells, using FAK (Phospho-Tyr925) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTK2-Tyr925 antibody (892-941 aa) (STJ90269)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from A-549 cells, using Phospho-FAK-Y397 Rabbit pAb (STJ27521) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-PTK2-Y397 antibody (STJ27521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of A-549, using Phospho-FAK-Y397 Rabbit monoclonal antibody (STJ11105895) at 1:4000 dilution. A-549 cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-PTK2-Y397 antibody [S5895RM] (STJ11105895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of various lysates, using Phospho-FAK-Y576/577 Rabbit pAb (STJ117876) at 1:1000 dilution. Jurkat cells were treated by CD-3 (10 Mu g/mL) for 2 minutes after serum-starvation overnight or treated by PMA/TPA (200nM) for 10 minutes. Raji cells were treated by PMA/TPA (200nM) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-PTK2-Y576/577 antibody (STJ117876)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Western blot analysis of lysates from Jurkat cells, using Phospho-FAK-Y925 Rabbit pAb (STJ29365) 1:1000 dilution.. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PTK2-Y925 antibody (STJ29365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Anti-Phospho-PTPN1-Ser-50 antibody (STJA0005553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

€459.38

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Western blot analysis of COLO cells using Phospho-PTP1B (S50) Polyclonal Antibody diluted at 1：500

Anti-Phospho-PTPN1-Ser50 antibody (16-65 aa) (STJ90882)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Anti-Phospho-PTPN1-Tyr-152 antibody (STJA0005554)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€459.38

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Western blot analysis of various cells using Phospho-SH-PTP2 (Y542) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-PTPN11-Tyr542 antibody (508-557 aa) (STJ90741)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-SH-PTP2 (Y580) Polyclonal Antibody

Anti-Phospho-PTPN11-Tyr580 antibody (546-595 aa) (STJ90742)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-SHP2-Y542 Rabbit pAb (STJ22358) at 1:700 dilution. NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-PTPN11-Y542 antibody (STJ22358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-Phospho-PTPN6-Ser-591 antibody (STJA0005582)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€459.38

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Anti-Phospho-PTPN6-Tyr-536 antibody (STJA0005581)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€459.38

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Western blot analysis of RAW264.7+EGF cells using Phospho-SH-PTP1 (Y536) Polyclonal Antibody

Anti-Phospho-PTPN6-Tyr536 antibody (502-551 aa) (STJ90740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using SHP-1 (Phospho-Tyr564) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-PTPN6-Tyr564 antibody (530-579 aa) (STJ90575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Monkey

€61.25 - €326.25

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Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using Phospho-SHP1-Y536 antibody (STJ22360).

Anti-Phospho-PTPN6-Y536 antibody (STJ22360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from HeLa and RAW 264. 7 cells, using Phospho-SHP1-Y564 Rabbit pAb (STJ117885) at 1:2000 dilution. HeLa cell lysates were treated by CIP at 37°C for 1 hour. RAW 264. 7 cell lysates were treated by CIP at 37°C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-PTPN6-Y564 antibody (STJ117885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€158.13 - €773.13

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Western blot analysis of lysates from A-431 cells, using Phospho-Rac1/2/3-S71 Rabbit pAb (STJ22364) at 1:1000 dilution. A-431 cells were treated by EGF (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-RAC1-S71 antibody (STJ22364)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded human placenta, using GTPase Activating Protein (Phospho-Ser387) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RACGAP1-Ser387 antibody (353-402 aa) (STJ91093)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Western blot analysis of lysates from HeLa cells, using Phospho-Raf1-S259 Rabbit pAb (STJ113482) at 1:1000 dilution. HeLa cells were treated by 10% FBS after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-RAF1-S259 antibody (STJ113482)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

€158.13 - €773.13

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