Gene Expression And Transcription | Research Area | St John's Laboratory

Western blot analysis of various lysates using PHC2 Rabbit polyclonal antibody (STJ116820) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PHC2 antibody (250-323) (STJ116820)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Immunofluorescence analysis of U2OS cells using PHC3 antibody (STJ116086). Blue: DAPI for nuclear staining.

Anti-PHC3 antibody (100-300) (STJ116086)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of extracts of various cell lines, using PHC3 Antibody (STJ28562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PHC3 antibody (100-300) (STJ28562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-PHC3 antibody (720-800 aa) (STJ191177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various lysates using PHF1 Rabbit pAb (STJ110748) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PHF1 antibody (1-260) (STJ110748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using PHF1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PHF1 antibody (345-394 aa) (STJ95062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using PHF19 Rabbit pAb (STJ110368) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PHF19 antibody (1-207) (STJ110368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Anti-PHF19 antibody (STJA0006961)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-PHF20 antibody (STJA0008246)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-PHF20L1 IP antibody [SAA0254] (STJA0018769)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

€473.13 - €2,040.63

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Western blot analysis of RAW264.7 cells using TDAG51 Polyclonal Antibody

Anti-PHLDA1 antibody (271-320 aa) (STJ95955)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Thr735 antibody (701-750 aa) (STJ90579)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Anti-Phospho-ABL1-Thr735 antibody (STJ196256)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Anti-Phospho-ABL1-Tyr-245 antibody (STJA0005239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€456.88

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Anti-Phospho-ABL1-Tyr-412 antibody (STJA0005240)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€456.88

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-ABL1-Tyr204 antibody (156-205 aa) (STJ90452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€61.25 - €326.25

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Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ABL1-Tyr245 antibody (196-245 aa) (STJ90694)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-Phospho-ABL1-Tyr245 antibody [PT1402R] (STJA0040363)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human

€187.50 - €675.00

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Anti-Phospho-ABL1-Tyr412 antibody (STJ196258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western Blot analysis of Hela treated or untreated by LPS lysis, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ABL1-Tyr89 antibody (STJ196259)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y245 Rabbit pAb (STJ22472). C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3S.

Anti-Phospho-ABL1-Y245 antibody (STJ22000)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from C6 cells, using Phospho-c-Abl-Y412 Rabbit pAb (STJ22472). C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-ABL1-Y412 antibody (STJ11101098)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma using Phospho-c-Abl-Y412 Rabbit pAb (STJ22003). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-ABL1-Y412 antibody (STJ22003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-Phospho-ACTB-Tyr-53 antibody (STJA0005242)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€456.88

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Anti-Phospho-AGO2-Ser-387 antibody (STJA0005264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€456.88

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Anti-Phospho-AGO2-Tyr-393 antibody (STJA0005265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

€456.88

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Western blot with Anti-Phospho-AKT1 Antibody (STJ502089) with PAKT peptide.1:250 dilution in buffer. From 10% SDS gel.

Anti-Phospho-AKT1 antibody (430-480) (STJ502089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

€660.63

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Anti-Phospho-AKT1 antibody (430-480) {Biotin} (STJ502090)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

€730.63

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Anti-Phospho-AKT1 antibody (430-480) {FITC} (STJ502091)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Bird/Bovine/Human/Mouse

€730.63

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Anti-Phospho-AKT1 antibody [M114] (STJA0005252)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€456.88

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S124 Rabbit mAb (STJ11102574) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S124 antibody [S4MR] (STJ11102574)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Western blot analysis of lysates from 293T cells, using Phospho-AKT1-S129 Rabbit pAb (STJ11103780) at 1:500 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-S129 antibody (STJ11103780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€158.13 - €773.13

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Western blot analysis of lysates from MCF7 cells, using Phospho-AKT1-S129 Rabbit mAb (STJ11102573) at 1:1000 dilution. MCF7 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-AKT1-S129 antibody [S3MR] (STJ11102573)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€148.13 - €651.88

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Anti-Phospho-AKT1-S212 antibody [SAA0279] (STJA0019075)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

€473.13 - €2,040.63

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€61.25 - €326.25

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Western blot analysis of lysates from C6 cells using Phospho-AKT1-S473 Rabbit pAb (STJ116369) at 1:400 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-AKT1-S473 antibody (STJ116369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Anti-Phospho-AKT1-S473 antibody [P8H9] (STJA0019074)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/AKT8 murine leukemia virus

€473.13 - €2,040.63

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Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€179.38 - €773.13

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Anti-Phospho-AKT1-S473 antibody [SAA0300] (STJA0019165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

€473.13 - €2,040.63

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-Akt1 (S129) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser129 antibody (95-144 aa) (STJ90484)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-Phospho-AKT1-Ser129 antibody (STJ99594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser246 antibody (212-261 aa) (STJ90165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from HeLa cells, using Phospho-AKT1-T308 Rabbit pAb (STJ11103747) at 1:1000 dilution. HeLa cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ11103747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-AKT1-T308 Rabbit pAb (STJ22013) at 1:600 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-AKT1-T308 antibody (STJ22013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €773.13

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Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€148.13 - €651.88

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Western blot analysis of lysates from RD cells, using Phospho-AKT1-T450 Rabbit pAb (STJ22008) at 1:600 dilution. RD cells were treated by Lambda-PP mixed solution (1ul) at 30 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-AKT1-T450 antibody (STJ22008)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€158.13 - €773.13

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