Gene Expression And Transcription | Research Area | St John's Laboratory

Immunofluorescence analysis of Hela cell. 1, E2F-1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HER2 monoclonal antibody (11H9) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ERBB2 antibody [11H9] (STJ96947)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of ERBB2 at dilution of 1:1000

Anti-ErbB2 antibody [SM0478] (STJA0010478)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£153.50 - £228.50

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Western blot analysis of extracts of various cell lines, using XPD/ERCC2 antibody (STJ116774) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ERCC2 antibody (1-180) (STJ116774)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells using XPD/XPD/ERCC2 antibody (STJ27607). Blue: DAPI for nuclear staining.

Anti-ERCC2 antibody (1-300) (STJ27607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Anti-ERCC2 antibody (220-300 aa) (STJ190429)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using ERCC3 Rabbit polyclonal antibody (STJ114575) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ERCC3 antibody (512-782) (STJ114575)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using TFIIH p89 monoclonal antibody against PC12, HepG2 cell lysate.

Anti-ERCC3 antibody (C-Term) (STJ98562)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Cow/Dog

£149.00 - £360.00

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ERCC6 antibody (141-190 aa) (STJ92493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human tonsil using ERCC6 Rabbit polyclonal antibody (STJ11100168) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ERCC6 antibody (700-800) (STJ11100168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-ERK1 antibody (C-Term) [M233] (STJA0041191)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IHC

Reactivity :

Human/Mouse/Rat

£579.50

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Anti-ERK1 antibody [1D10-8A3-8C6] (STJA0032924)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-ERK1 antibody [7E7-10H1-7B6] (STJA0032922)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-ERK1 antibody [8G6-7F4-1G8] (STJA0032923)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human/Rat/Mouse

£133.50 - £281.50

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Anti-ERK1 antibody [R07-5H6] (STJA0035773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-ERK1 antibody [R08-3C6] (STJA0034289)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human

£133.50 - £281.50

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Anti-ERK1/MAPK3 antibody (1-137aa) [AT1A2] (STJA0041595)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-ERK2 antibody [R07-1A9] (STJA0033884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-ERK2/MAPK1 antibody (1-360aa) [AT1A4] (STJA0041589)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF

Reactivity :

Human

£219.50 - £332.50

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Anti-ERR alpha antibody [R04-5A7] (STJA0035997)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-ESR Alpha antibody [SRM14746] (STJ11109476)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA/ChIP/FC

Reactivity :

Human

£0.00

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Immunofluorescence analysis of NIH/3T3 using ESR Alpha Rabbit monoclonal antibody (STJ11104746) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ESR1 antibody (100-200) [S4746RM] (STJ11104746)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Western blot analysis of various cells using ER Alpha Polyclonal Antibody diluted at 1：500

Anti-ESR1 antibody (136-185 aa) (STJ92997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot with Anti-ESR1 Antibody (STJ500969) and peptide at 1:500 dilution in buffer.

Anti-ESR1 antibody (30-80) (STJ500969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£400.50

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Anti-ESR1 antibody (30-80) {Biotin} (STJ500972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£497.50

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Anti-ESR1 antibody (30-80) {FITC} (STJ500973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£497.50

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Human breast carcinoma tissue was stained with Anti-Estrogen Receptor α (PTR2124) Antibody

Anti-ESR1 antibody (450-595) [PTR2124] (STJA0006244)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ELISA

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of lysates from MCF7 cells, using ESR Alpha Rabbit monoclonal antibody (STJ114945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-ESR1 antibody (50-150) [S5MR] (STJ114945)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-ESR1 antibody (501-550 aa) (STJ92999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of HeLa cells, using ESR Alpha antibody (STJ23572) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ESR1 antibody (516-595) (STJ23572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Anti-ESR1 antibody (520-570) (STJ500970)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£400.50

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Anti-ESR1 antibody (520-570) {Biotin} (STJ500974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£497.50

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Anti-ESR1 antibody (520-570) {FITC} (STJ500975)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Rat

£497.50

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Immunohistochemistry of mouse liver with Anti-ESR1 primary antibody (STJ500971) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-ESR1 antibody (545-595) (STJ500971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£400.50

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Anti-ESR1 antibody (545-595) {Biotin} (STJ500976)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£497.50

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Anti-ESR1 antibody (545-595) {FITC} (STJ500977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£497.50

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Anti-ESR1 antibody (C-Term) (STJA0005414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

£319.50

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Western blot analysis of 1) Hela, 2) MCF7, 3) Mouse Liver Tissue, 4) Rat Liver Tissue with Estrogen Receptor a Rabbit pAb diluted at 1:2,000.

Anti-ESR1 antibody (STJ197393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis using ER monoclonal antibody against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ER cDNA (2).

Anti-ESR1 antibody [5D4B1/5D4E3] (STJ98040)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis using ER Alpha monoclonal antibody against MCF-7 (1) , T47D (2) and SKBR3 (3) cell lysate.

Anti-ESR1 antibody [6B6] (STJ98054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-ESR1/ER-alpha antibody [RB125] (STJA0022317)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF

Reactivity :

Human

£195.50 - £1,319.50

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Anti-ESR1/ER-alpha antibody [RB133] (STJA0022318)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£195.50 - £1,319.50

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Western blot analysis of lysates from Daudi cells using Estrogen Receptor beta Rabbit polyclonal antibody (STJ23576) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ESR2 antibody (1-153) (STJ23576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-ER-β (PT0318) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: HEK293 Lane 3: MCF7Predicted band size: 59kDaObserved band size: 59kDa

Anti-ESR2 antibody (400-530 aa) (STJ196169)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human

£49.00 - £261.00

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Immunofluorescence analysis of mouse-kidney tissue. 1, ER Beta Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-ESR2 antibody (71-120 aa) (STJ93002)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using ESRRA Rabbit monoclonal antibody (STJ11102011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ESRRA antibody (1-100) [S1MR] (STJ11102011)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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Western blot analysis of extracts of various cell lines, using ESRRA antibody (STJ116117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ESRRA antibody (1-76) (STJ116117)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using ESRRA antibody (STJ23577) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-ESRRA antibody (1-76) (STJ23577)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ESRRA antibody (160-240 aa) (STJ192507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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