Extracellular Matrix Organisation | Research Area | St John's Laboratory

Western blot analysis of various lysates using Collagen I/COL1A1 rabbit polyclonal antibody (STJ111079) at 1:4000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-COL1A1 antibody (1189-1205) (STJ111079)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunocytochemistry analysis of Collagen I alpha 1 (green) in Hela using Collagen I alpha 1 antibody, and DAPI (blue).

Anti-COL1A1 antibody (1208-1453 aa) [R02-5E5] (STJA0013087)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human

€330.63

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Immunofluorescence analysis of NIH/3T3 cells using COL1A1 Rabbit polyclonal antibody (STJ11105651) at a dilution of 1:250 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A1 antibody (1359-1464) (STJ11105651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunofluorescence analysis of paraffin-embedded human colon using Human Pro-Collagen I alpha Rabbit polyclonal antibody (STJ11105505) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-COL1A1 antibody (26-161) (STJ11105505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC

Reactivity :

Human

€138.13 - €631.88

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Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, Collagen I Mouse monoclonal antibody (4H10) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-COL1A1 antibody (STJ98915)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IHC

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-COL1A1 nanobody [SAA1295] (STJN000085)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Immunofluorescence analysis of human-stomach tissue. 1, COL1A2 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-COL1A2 antibody (1-50 aa) (STJ92383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of various lysates using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ119111) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-COL1A2 antibody (1161-1260) (STJ119111)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various cells using COL1A2 Polyclonal Antibody diluted at 1：1000

Anti-COL1A2 antibody (471-520 aa) (STJ92384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A2 Rabbit polyclonal antibody (STJ28351) at dilution of 1:25. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL1A2 antibody (501-600) (STJ28351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of various lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-COL1A2 antibody (STJ99633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Immunofluorescence analysis of NIH/3T3 cells, using Collagen XX alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL20A1 antibody (1151-1200 aa) (STJ92396)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from A549 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-COL21A1 antibody (101-151 aa) (STJ193704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Immunofluorescence analysis of HepG2 cells, using Collagen XXIII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL23A1 antibody (461-510 aa) (STJ92385)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from Jurkat and A549 cells, using Collagen XXV alpha1 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-COL25A1 antibody (101-150 aa) (STJ92386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of HEPG2 cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-COL25A1 antibody (101-150 aa) (STJ98655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-COL25A1 antibody (NC2-1 region) (STJA0005754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€569.38

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Anti-COL25A1 antibody (NC2-2 region) (STJA0005755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€569.38

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Anti-COL25A1 antibody (NC3 region) (STJA0005756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€569.38

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Anti-COL26A1 antibody (120-200 aa) (STJ191843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Anti-COL27A1 antibody (1551-1600 aa) (STJ192567)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of 3T3 HEPG2 cells using Collagen alpha-1 (XXVIII) Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-COL28A1 antibody (111-160 aa) (STJ98615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of lysates from 1) k562, 2) COLO205, 3) MCF-7 cells, （Green） primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. （Red） Actin Beta monoclonal antibody (5B7) (cat: (STJ96930) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-COL2A1 antibody (101-150 aa) (STJ92387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Human cartilage tissue was stained with Anti-Collagen Type II (ABT501) Antibody

Anti-COL2A1 antibody (1200-1300 aa) [ABT501] (STJ197134)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€225.00 - €326.25

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Immunofluorescence analysis of HepG2 cells using COL2A1 Rabbit polyclonal antibody (STJ23191) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL2A1 antibody (1220-1282) (STJ23191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-COL2A1 antibody (STJ98944)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Anti-COL2A1 antibody [ACC2/3] (STJA0019070)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-COL2A1 antibody [GB8] (STJA0019071)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Anti-COL2A1 antibody [UL1] (STJA0019072)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

€473.13 - €2,040.63

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Human colon tissue was stained with Anti-Collagen Type III (ABT-CIII) Antibody

Anti-COL3A1 antibody (100-200 aa) [ABT-CIII] (STJ196885)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

€0.00

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Immunohistochemical analysis of paraffin-embedded Skin. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-COL3A1 antibody (100-200 aa) [PT0118] (STJ196955)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€0.00

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Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 Rabbit polyclonal antibody (STJ23192) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-COL3A1 antibody (1201-1301) (STJ23192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€138.13 - €631.88

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Immunocytochemistry analysis of Collagen III (green) in Hela using Collagen III antibody, and DAPI (blue)

Anti-COL3A1 antibody (201-400 aa) [R06-4E1] (STJA0012676)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

€330.63

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Immunofluorescence analysis of HeLa cells, using Collagen III Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL3A1 antibody (81-130 aa) (STJ92388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of lysates from 1) MCF7, 2) Hela, 3) 293T cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (cat: (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.

Anti-COL3A1 antibody [Q76] (STJ97025)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of mouse-musle cells using COL4A1 Polyclonal Antibody diluted at 1：500

Anti-COL4A1 antibody (11-60 aa) (STJ92389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of Hela MCF-7 U2OS 293T KB 293T VEC cells using Collagen IV Polyclonal Antibody diluted at 1:800. Secondary antibody was diluted at 1:20000

Anti-COL4A1 antibody (1428-1443 aa) (STJ98599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€61.25 - €326.25

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Western blot analysis of extracts of U-87 MG cells, using COL4A1 Antibody (STJ112627) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 30s.

Anti-COL4A1 antibody (1445-1669) (STJ112627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Western blot analysis of HeLa, using COL4A2 Rabbit polyclonal antibody (STJ29971) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-COL4A2 antibody (1486-1712) (STJ29971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Western blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1：2000

Anti-COL4A2 antibody (151-200 aa) (STJ92390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of extracts of mouse kidney, using COL4A3 antibody (STJ118787) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-COL4A3 antibody (1555-1669) (STJ118787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€138.13 - €631.88

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Immunofluorescence analysis of COS7 cells, using Collagen IV alpha3 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A3 antibody (801-850 aa) (STJ92391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Western blot analysis of 293F, using COL4A4 antibody (STJ11104960) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-COL4A4 antibody (1573-1690 aa) (STJ11104960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€138.13 - €631.88

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Immunofluorescence analysis of COS7 cells, using Collagen IV alpha4 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A4 antibody (541-590 aa) (STJ92392)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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Anti-COL4A4/COL4A6/COL4A2/COL4A1 antibody (1573-1690 aa) [SRM5263] (STJ11107165)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human/Mouse/Rat

€158.13 - €693.13

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Western blot analysis of various lysates using COL4A5 Rabbit pAb (STJ111851) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-COL4A5 antibody (1462-1691) (STJ111851)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€138.13 - €631.88

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Immunofluorescence analysis of HeLa cells, using Collagen IV alpha5 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-COL4A5 antibody (21-70 aa) (STJ92393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

€61.25 - €326.25

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Western blot analysis of various cells using COL4A6 Polyclonal Antibody diluted at 1：500

Anti-COL4A6 antibody (1201-1250 aa) (STJ92394)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€61.25 - €326.25

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