Developmental Biology | Research Area | St John's Laboratory

Immunoprecipitation analysis of 200 Mu g extracts of HepG2 cells using 1 Mu g POLR2D antibody (STJ25047). Western blot was performed from the immunoprecipitate using POLR2D antibody (STJ25047) at a dilution of 1:1000.

Anti-POLR2D antibody (1-142) (STJ25047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2D antibody (STJ190763)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$67.62 - $360.18

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Anti-POLR2D antibody [SRM13432] (STJ11108162)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of A549 cells using POLR2E Rabbit polyclonal antibody (STJ25048). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-POLR2E antibody (1-210) (STJ25048)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-POLR2E antibody (21-70 aa) (STJ95176)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from rat heart, using POLR2F Rabbit polyclonal antibody (STJ116307) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLR2F antibody (1-127) (STJ116307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2F antibody (STJ190771)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using POLR2G antibody (STJ28920) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-POLR2G antibody (1-172) (STJ28920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2G antibody (STJ190764)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g POLR2H antibody (STJ115302). Western blot was performed from the immunoprecipitate using POLR2H antibody (STJ115302) at a dilution of 1:1000.

Anti-POLR2H antibody (1-150) (STJ115302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse

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Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g POLR2H antibody (STJ29028). Western blot was performed from the immunoprecipitate using POLR2H antibody (STJ29028) at a dilution of 1:1000.

Anti-POLR2H antibody (1-150) (STJ29028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2H antibody (STJ190772)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts of various cell lines, using POLR2I antibody (STJ25051) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-POLR2I antibody (1-125) (STJ25051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-POLR2I antibody (STJ190765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

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Immunofluorescence analysis of A549 cells using POLR2J antibody (STJ25052).

Anti-POLR2J antibody (1-117) (STJ25052)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of lysates from RAW264.7 cells, using RPB11 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-POLR2J antibody (10-59 aa) (STJ95177)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-POLR2K antibody (1-50 aa) (STJ196497)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

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Anti-POLR2L antibody (1-67 aa) [SRM5426] (STJ11107339)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$198.03 - $853.53

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Western blot analysis of various lysates, using POLR2L rabbit polyclonal antibody (STJ25053) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POLR2L antibody (1-67) (STJ25053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-POLR2L antibody (STJA0009361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

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Anti-POLR2L antibody [SRM14855] (STJ11109585)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of 293F-POMC-His-C, using POMC rabbit polyclonal antibody (STJ25054) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POMC antibody (1-100) (STJ25054)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Human pituitary tissue was stained with anti-ACTH (ABT-ACTH) antibody.

Anti-POMC antibody (100-200 aa) [ABT-ACTH] (STJ196740)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

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Anti-POMC antibody (STJ193173)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using POU3F1 Rabbit polyclonal antibody (STJ11100601) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POU3F1 antibody (1-260) (STJ11100601)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

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Western blot analysis of various cells using Oct-6 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-POU3F1 antibody (311-360) (STJ94599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using POU3F2 Rabbit polyclonal antibody (STJ11100526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-POU3F2 antibody (1-68) (STJ11100526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

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Anti-POU3F2 antibody (STJ191936)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-POU3F3 antibody (90-170 aa) (STJ190442)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

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Human seminoma stained with anti-OCT-4 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of seminoma cells.

Anti-POU5F1 antibody (1-134aa) [C‐10] (STJ180200)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P

Reactivity :

Human

$222.87 - $686.55

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Western blot analysis of various cells using Oct-3/4 Polyclonal Antibody diluted at 1：1000

Anti-POU5F1 antibody (191-240) (STJ94598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of lysates from Mouse embryo, using OCT4 Rabbit pAb (STJ110229) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-POU5F1 antibody (210-310) (STJ110229)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

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Western blot analysis using Oct-3/4 monoclonal antibody against F9 cell lysate.

Anti-POU5F1 antibody (STJ98523)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/IF

Reactivity :

Human

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Western blot detection of POU5F1/OCT4 in F9 cell lysates using POU5F1/OCT4 mouse mAb (1:1000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.

Anti-POU5F1 antibody [3F5-F9-E5] (STJ99042)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

$205.62 - $496.80

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Western blot analysis using Oct-3/4 monoclonal antibody against NTERA-2 (1) cell lysate.

Anti-POU5F1 antibody [7E7] (STJ98287)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/FC/ELISA

Reactivity :

Human

$205.62 - $496.80

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Anti-PPAR Gamma antibody (STJ11107753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of various lysates using PPAR Alpha Rabbit pAb (STJ28780) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-PPARA antibody (1-92) (STJ28780)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

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Western blot analysis of lysates from Mouse kidney using PPARA Rabbit polyclonal antibody (STJ11105478) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PPARA antibody (201-300) (STJ11105478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Mouse

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Immunofluorescence analysis of Hela cell. 1, PPAR-Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Galectin-3 monoclonal antibody (6G2) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-PPARA antibody (6-55 aa) (STJ95200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of U2OS cells using PPAR Gamma Rabbit polyclonal antibody (STJ25069) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PPARG antibody (115-132) (STJ25069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using PPAR Gamma antibody (STJ11101758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PPARG antibody (50-150) [S8MR] (STJ11101758)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

$174.57 - $765.21

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Immunofluorescence analysis of rat-lung tissue. 1, PPAR-Gamma Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-PPARG antibody (78-127 aa) (STJ95201)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis using PPAR-Gamma monoclonal antibody against 3T3-L1, mouse lung cell lysate.

Anti-PPARG antibody (C-Term) (STJ98538)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Cow/Dog/Goat/Pig/Rabbit/Sheep

$205.62 - $496.80

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Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using PPAR γ mouse Monoclonal antibody diluted at 1:200.

Anti-PPARG antibody [Mix] (STJ196657)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$248.40 - $360.18

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Western blot analysis of various lysates, using PGC1 Alpha rabbit polyclonal antibody (STJ114227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PPARGC1A antibody (610-798) (STJ114227)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of various cell lines, using PGC1 Alpha antibody (STJ118021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PPARGC1A antibody (610-798) (STJ118021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis using PGC-1 Alpha monoclonal antibody against HEK293 (1) and PGC-1alpha-hIgGFc transfected HEK293 (2) cell lysate.

Anti-PPARGC1A antibody [1G8] (STJ98321)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$205.62 - $496.80

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-PPARGC1B antibody (829-879 aa) (STJ196101)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

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