Autophagy | Research Area | St John's Laboratory

Western blot analysis of 293 cells using AMPK Gamma 1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PRKAG1 antibody (10-59 aa) (STJ91585)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using AMPK Gamma 1 Rabbit polyclonal antibody (STJ11104668) at 1:500 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 90s.

Anti-PRKAG1 antibody (100-200) (STJ11104668)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of K562 using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-PRKAG2 antibody (1-50 aa) (STJ91587)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of RD cells using PRKAG3 Rabbit polyclonal antibody (STJ116067) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAG3 antibody (1-70) (STJ116067)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of NIH/3T3 cells using Parkin Rabbit polyclonal antibody (STJ113001) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-PRKN antibody (1-465) (STJ113001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Parkin Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PRKN antibody (1-50 aa) (STJ94956)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Parkin Polyclonal Antibody

Anti-PRKN antibody (101-150 aa) (STJ94958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Western blot with Anti-PRKN Antibody (STJ502119) on SKNMC cell at 1:500 dilution in buffer.

Anti-PRKN antibody (390-440) (STJ502119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£427.50

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Anti-PRKN antibody (390-440) {Biotin} (STJ502120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Anti-PRKN antibody (390-440) {FITC} (STJ502121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human

£528.50

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Immunofluorescence analysis of paraffin-embedded rat brain using Parkin Rabbit polyclonal antibody (STJ24894) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01M citrate buffer (pH 6. 0) prior to IF staining.

Anti-PRKN antibody (81-280) (STJ24894)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-PRKN antibody (N-Term) (STJA0005802)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£455.50

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Western blot analysis of extracts of various cell lines, using FIP200 rabbit polyclonal antibody (STJ116887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-RB1CC1 antibody (1280-1400) (STJ116887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-RB1CC1 antibody (STJA0006735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using RheB antibody (STJ25350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RHEB antibody (1-180) (STJ25350)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of mouse-kidney mouse-brain cells using RHEB Polyclonal Antibody diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-RHEB antibody (STJ98588)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of mouse pancreas, using RNASE1 antibody (STJ27958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-RNASE1 antibody (29-156) (STJ27958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using RPS27A antibody (STJ116826) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-RPS27A antibody (1-156) (STJ116826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using RPS27A antibody (STJ25406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPS27A antibody (1-156) (STJ25406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-RPS27A antibody (STJA0007619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RPTOR antibody (730-810 aa) (STJ190148)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using Raptor antibody (STJ11104521) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RPTOR antibody (888-1013) (STJ11104521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using Raptor Rabbit polyclonal antibody (STJ11104522) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer:3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-RPTOR antibody (888-1013) (STJ11104522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using Raptor Rabbit polyclonal antibody (STJ118739) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-RPTOR antibody (888-1013) (STJ118739)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of C6 cells using RRAGA Rabbit polyclonal antibody (STJ110082) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-RRAGA antibody (213-313) (STJ110082)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of RRAGA in paraffin-embedded human placenta using RRAGA Rabbit polyclonal antibody (STJ117328) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-RRAGA antibody (213-313) (STJ117328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-RRAGB antibody (50-130 aa) (STJ95356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Rag C Polyclonal Antibody

Anti-RRAGC antibody (302-351 aa) (STJ95357)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC/CoIP

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-RRAGD antibody (140-220 aa) (STJ191268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/CoIP

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of various lysates using RRAGD Rabbit pAb (STJ112020) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-RRAGD antibody (181-400) (STJ112020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Hela cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SLC38A9 antibody (264-314 aa) (STJ193549)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ110069) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody (1-440) (STJ110069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g SQSTM1/p62 antibody (STJ113162). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113162) at a dilution of 1:1000.

Anti-SQSTM1 antibody (1-440) (STJ113162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-SQSTM1 antibody (300-360 aa) (STJ13101971)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-SQSTM1 antibody (300-360 aa) (STJ13101972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-SQSTM1 antibody (300-360 aa) (STJ13102022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Anti-SQSTM1 antibody (300-360 aa) (STJ13102023)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Human

£331.50

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Immunohistochemistry of rat tissue with Anti-SQSTM1 primary antibody (STJ503125) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SQSTM1 antibody (400-440) (STJ503125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-SQSTM1 antibody (400-440) {Biotin} (STJ503126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-SQSTM1 antibody (400-440) {FITC} (STJ503127)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SQSTM1 antibody (STJ195471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TBK1 antibody (138-187 aa) (STJ95926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded rat testis using TBK1/NAK Rabbit polyclonal antibody (STJ25781) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-TBK1 antibody (500-600) (STJ25781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using TOM20 Rabbit polyclonal antibody (STJ28857) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TOMM20 antibody (1-145) (STJ28857)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using Tom20 Polyclonal Antibody diluted at 1：500. Secondary antibody was diluted at 1:20000

Anti-TOMM20 antibody (15-64 aa) (STJ96061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using [KO Validated] TOM20 Rabbit polyclonal antibody (STJ119224) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-TOMM20 antibody (20-145) (STJ119224)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various lysates using TOMM22 Rabbit polyclonal antibody (STJ116759) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TOMM22 antibody (1-83) (STJ116759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-TOMM22 antibody (STJA0007196)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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