• Rat SYP (Synaptophysin) CLIA Kit (STJC0001891)

Rat SYP (Synaptophysin) CLIA Kit (STJC0001891)

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STJC0001891

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 75pg/mL
Detection Limit: 125~8000pg/mL
Short Description: This rat SYP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Syp
Gene ID: 24804
Uniprot ID: SYPH_RAT
Specificity: This kit recognizes Rat SYP in samples. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the brain with expression in the cerebrum and the cerebellum.
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. Phosphorylated by SRC.
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity.
Protein Name Synaptophysin
Major Synaptic Vesicle Protein P38
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Multi-Pass Membrane Protein
Synapse
Synaptosome
Alternative CLIA Names Synaptophysin CLIA kit
Major Synaptic Vesicle Protein P38 CLIA kit
Syp CLIA kit
Specificity This kit recognizes Rat SYP in samples. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-103 94
EDTA plasma(n=8) 94-108 101
Cell culture media(n=8) 93-108 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 422.69 1221.32 2994.17 410.13 1268.11 2816.02
Standard deviation 54.06 111.51 277.26 48.27 116.54 202.47
CV (%) 12.79 9.13 9.26 11.77 9.19 7.19
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance