• Rat/Porcine E2 (Estradiol) Competitive ELISA (STJE0006571)

Rat/Porcine E2 (Estradiol) Competitive ELISA (STJE0006571)

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STJE0006571

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Applications: ELISA
Reactivity: Rat/Porcine
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.00pg/mL
Detection Limit: 1.56~100pg/mL
Short Description: This E2 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat/porcine cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Specificity: This kit recognizes Rat/Porcine E2 in samples. No significant cross-reactivity or interference between Rat/Porcine E2 and analogues was observed.
Sample Type: Serum and plasma
Specificity This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 80-91 84
EDTA plasma(n=8) 82-93 87
Urine(n=8) 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 40.33 90.22 200.31 41.25 92.32 201.59
Standard deviation 3.52 6.80 10.67 3.76 7.36 12.55
CV (%) 8.75 7.54 5.33 9.12 7.98 6.23
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance