• Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit (STJC0001838)

Rat PAI-1 (Plasminogen Activator Inhibitor 1) CLIA Kit (STJC0001838)

SKU:
STJC0001838

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This rat PAI-1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Serpine1
Gene ID: 24617
Uniprot ID: PAI1_RAT
Specificity: This kit recognizes Rat PAI-1 in samples. No significant cross-reactivity or interference between Rat PAI-1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PLAT) and urokinase-type plasminogen activator (PLAU). As PLAT inhibitor, it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PLAU inhibitor, it is involved in the regulation of cell adhesion and spreading. Acts as a regulator of cell migration, independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells, and regulates odontoblast differentiation and dentin formation during odontogenesis.
Protein Name Plasminogen Activator Inhibitor 1
Pai
Pai-1
Endothelial Plasminogen Activator Inhibitor
Serpin E1
Database Links Reactome: R-RNO-114608
Reactome: -RNO-3000178
Reactome: -RNO-75205
Cellular Localisation Secreted
Alternative CLIA Names Plasminogen Activator Inhibitor 1 CLIA kit
Pai CLIA kit
Pai-1 CLIA kit
Endothelial Plasminogen Activator Inhibitor CLIA kit
Serpin E1 CLIA kit
Serpine1 CLIA kit
Pai1 CLIA kit
Planh1 CLIA kit
Specificity This kit recognizes Rat PAI-1 in samples. No significant cross-reactivity or interference between Rat PAI-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-111 102
EDTA plasma(n=8) 86-98 92
Cell culture media(n=8) 95-110 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 204.05 466.61 1710.00 195.09 481.94 1692.65
Standard deviation 25.00 47.22 141.25 22.63 43.57 141.67
CV (%) 12.25 10.12 8.26 11.60 9.04 8.37
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance