| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of 50mM Tris, 10mM CaCl2, 150mM NaCl, pH 7.5. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | 1.Measured in a cell migration assay using Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration.|2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is > |
| Gene Symbol: | Mmp9 |
| Gene ID: | 81687 |
| Uniprot ID: | MMP9_RAT |
| Immunogen Region: | Leu35-Pro708 |
| Immunogen: | Recombinant Rat MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu35-Pro708) of rat MMP-9 (Accession #NP_038627.1.) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | LKTSNLTDTQLAEDYLYRYG YTRAAQMMGEKQSLRPALLM LQKQLSLPQTGELDSETLKA IRSPRCGVPDVGKFQTFEGD LKWHHHNITYWIQSYTEDLP RDVIDDSFARAFAVWSAVTP LTFTRVYGLEADIVIQFGVA EHGDGYPFDGKDGLLAHAFP PGPGIQGDAHFDDDELWSLG KGAVVPTYFGNANGAPCHFP FTFEGRSYLSCTTDGRNDGK PWCGTTADYDTDRKYGFCP |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. |
| Protein Name | Matrix Metalloproteinase-9Mmp-992 Kda Gelatinase92 Kda Type Iv CollagenaseGelatinase BGelb |
| Database Links | Reactome: R-RNO-1433557Reactome: -RNO-1442490Reactome: -RNO-1474228Reactome: -RNO-1592389Reactome: -RNO-2022090Reactome: -RNO-3928665Reactome: -RNO-6798695Reactome: -RNO-9009391 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Matrix Metalloproteinase-9 proteinMmp-9 protein92 Kda Gelatinase protein92 Kda Type Iv Collagenase proteinGelatinase B proteinGelb proteinMmp9 protein |
Information sourced from Uniprot.org
