Rat MMP-9 protein (Recombinant) (C-His) (STJP001226)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Rat MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu35-Pro708) of rat MMP-9 (Accession #NP_038627.1.) fused with a 6ΓHis tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat MMP-9-C-His protein was developed in hek293 cells using the region Leu35-Pro708. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 50mM Tris, 10mM CaCl2, 150mM NaCl, pH 7.5. |
| Storage Instruction | Store at-20Β°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured in a cell migration assay using Hela cells. 1 ng/mL of Recombinant Rat MMP-9 can effectively induce Hela cells migration.|2.Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The specific activity is > |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Mmp9 |
| Gene ID | 81687 |
| Uniprot ID | MMP9_RAT |
| Immunogen | Recombinant Rat MMP-9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu35-Pro708) of rat MMP-9 (Accession #NP_038627.1.) fused with a 6ΓHis tag at the C-terminus. |
| Immunogen Region | Leu35-Pro708 |
| Immunogen Sequence | LKTSNLTDTQLAEDYLYRYG YTRAAQMMGEKQSLRPALLM LQKQLSLPQTGELDSETLKA IRSPRCGVPDVGKFQTFEGD LKWHHHNITYWIQSYTEDLP RDVIDDSFARAFAVWSAVTP LTFTRVYGLEADIVIQFGVA EHGDGYPFDGKDGLLAHAFP PGPGIQGDAHFDDDELWSLG KGAVVPTYFGNANGAPCHFP FTFEGRSYLSCTTDGRNDGK PWCGTTADYDTDRKYGFCP |
Additional Info
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. |
| Protein Name | Matrix Metalloproteinase-9Mmp-992 Kda Gelatinase92 Kda Type Iv CollagenaseGelatinase BGelb |
| Database Links | Reactome: R-RNO-1433557Reactome: -RNO-1442490Reactome: -RNO-1474228Reactome: -RNO-1592389Reactome: -RNO-2022090Reactome: -RNO-3928665Reactome: -RNO-6798695Reactome: -RNO-9009391 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Protein Names | Matrix Metalloproteinase-9 proteinMmp-9 protein92 Kda Gelatinase protein92 Kda Type Iv Collagenase proteinGelatinase B proteinGelb proteinMmp9 protein |
Information sourced from Uniprot.org