Rat Kiss1 (Metastasis-suppressor KiSS-1) Sandwich ELISA Kit (STJE0006473)

Name: Rat Kiss1 (Metastasis-suppressor KiSS-1) Sandwich ELISA Kit (STJE0006473)
SKU: STJE0006473
Availability: InStock

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SPECIFICATIONS

STJE0006473

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Short Description	This Kiss1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	46.88pg/mL
Detection Limit	78.13~5000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Kiss1
Gene ID	289023
Uniprot ID	KISS1_RAT
Specificity	This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.
Protein Name	Metastasis-Suppressor Kiss-1 Kisspeptin-1 Cleaved Into - Metastin - Kisspeptin-10 Metastin45-54
Database Links	Reactome: R-RNO-375276 Reactome: -RNO-416476
Cellular Localisation	Secreted
Alternative ELISA Names	Metastasis-Suppressor Kiss-1 ELISA kit Kisspeptin-1 Cleaved Into - Metastin - Kisspeptin-10 ELISA kit Metastin45-54 ELISA kit Kiss1 ELISA kit
Specificity	This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	228.82	578.41	2346.06	244.62	578.38	2521.30
Standard deviation	13.41	32.04	79.30	15.78	27.94	92.28
CV (%)	5.86	5.54	3.38	6.45	4.83	3.66