Rat Il1b (Interleukin-1 beta) Sandwich ELISA Kit (STJE0005440)

Name: Rat Il1b (Interleukin-1 beta) Sandwich ELISA Kit (STJE0005440)
SKU: STJE0005440
Availability: InStock

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SPECIFICATIONS

STJE0005440

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Short Description	This Il1b Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Rat
Sensitivity	18.75pg/mL
Detection Limit	31.25~2000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Il1bUniprot ID=IL1B_RAT"
Specificity	This kit recognizes Rat IL-1 Beta in samples. No significant cross-reactivity or interference between Rat IL-1 Beta and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Protein Name	Interleukin-1 Beta Il-1 Beta
Cellular Localisation	Cytoplasm Cytosol Secreted Lysosome Extracellular Exosome The Precursor Is Cytosolic In Response To Inflammasome-Activating Signals Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome Cleaved And Secreted Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore In Contrast The Precursor Form Is Not Released Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
Alternative ELISA Names	Interleukin-1 Beta ELISA kit Il-1 Beta ELISA kit Il1b ELISA kit
Specificity	This kit recognizes Rat IL-1β in samples. No significant cross-reactivity or interference between Rat IL-1β and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	100.50	314.20	880.10	107.40	340.60	954.80
Standard deviation	5.70	13.50	30.80	5.60	20.40	33.40
CV (%)	5.67	4.30	3.50	5.21	5.99	3.50