Rat Fgf23 (Fibroblast growth factor 23) Sandwich ELISA Kit (STJE0006566)
SPECIFICATIONS
General Information
| Short Description | This Fgf23 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 37.5pg/mL |
| Detection Limit | 62.5~4000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | Fgf23 |
| Gene ID | 170583 |
| Uniprot ID | FGF23_RAT |
| Specificity | This kit recognizes Rat FGF23 in samples. No significant cross-reactivity or interference between Rat FGF23 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion.it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably. |
| Function | Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Regulator of vitamin-D metabolism. Negatively regulates osteoblasts differentiation and matrix mineralization. Acts directly on the parathyroid to decrease PTH secretion. Up-regulates EGR1 expression in the presence of KL. |
| Protein Name | Fibroblast Growth Factor 23Fgf-23 |
| Database Links | Reactome: R-RNO-109704Reactome: -RNO-1257604Reactome: -RNO-190322Reactome: -RNO-190372Reactome: -RNO-190373Reactome: -RNO-190374Reactome: -RNO-190375Reactome: -RNO-381426Reactome: -RNO-5654219Reactome: -RNO-5654221Reactome: -RNO-5654227Reactome: -RNO-5654228Reactome: -RNO-5654687Reactome: -RNO-5654688Reactome: -RNO-5654689Reactome: -RNO-5654693Reactome: -RNO-5654695Reactome: -RNO-5654699Reactome: -RNO-5654700Reactome: -RNO-5654704Reactome: -RNO-5654706Reactome: -RNO-5654710Reactome: -RNO-5654712Reactome: -RNO-5654719Reactome: -RNO-5654720Reactome: -RNO-5654726Reactome: -RNO-5654727Reactome: -RNO-5654732Reactome: -RNO-5654733Reactome: -RNO-5658623Reactome: -RNO-5673001Reactome: -RNO-6811558Reactome: -RNO-8957275 |
| Cellular Localisation | SecretedSecretion Is Dependent On O-Glycosylation |
| Alternative ELISA Names | Fibroblast Growth Factor 23 ELISA kitFgf-23 ELISA kitFgf23 ELISA kit |
| Specificity | This kit recognizes Rat FGF23 in samples. No significant cross-reactivity or interference between Rat FGF23 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 94-105 | 102 |
| EDTA plasma (n=8) | 96-111 | 104 |
| Cell culture media (n=8) | 89-95 | 92 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 190.98 | 446.20 | 1851.75 | 192.40 | 482.24 | 1710.53 |
| Standard deviation | 10.92 | 20.57 | 98.70 | 10.04 | 21.89 | 82.79 |
| CV (%) | 5.72 | 4.61 | 5.33 | 5.22 | 4.54 | 4.84 |