• Rat DRD4 (Dopamine Receptor D4) CLIA Kit (STJC0001654)

Rat DRD4 (Dopamine Receptor D4) CLIA Kit (STJC0001654)

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STJC0001654

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This rat DRD4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Drd4Uniprot ID=DRD4_RAT"
Specificity: This kit recognizes Rat DRD4 in samples. No significant cross-reactivity or interference between Rat DRD4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Central nervous system: hypothalamus, thalamus, olfactory bulb, and frontal cortex.
Post Translational Modifications Polyubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex: polyubiquitination does not lead to degradation of DRD4 protein. Palmitoylated. Palmitoylation of the C-terminal Cys is important for normal expression at the cell membrane.
Function Dopamine receptor responsible for neuronal signaling in the mesolimbic system of the brain, an area of the brain that regulates emotion and complex behavior. Activated by dopamine, but also by epinephrine and norepinephrine, and by numerous synthetic agonists and drugs. Agonist binding triggers signaling via G proteins that inhibit adenylyl cyclase. Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells.
Protein Name D(4 Dopamine Receptor
D(2c Dopamine Receptor
Dopamine D4 Receptor
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative CLIA Names D(4 Dopamine Receptor CLIA kit
D(2c Dopamine Receptor CLIA kit
Dopamine D4 Receptor CLIA kit
Drd4 CLIA kit
Specificity This kit recognizes Rat DRD4 in samples. No significant cross-reactivity or interference between Rat DRD4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-114 107
EDTA plasma(n=8) 94-108 101
Cell culture media(n=8) 87-100 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 94.73 214.58 774.08 98.17 195.37 839.44
Standard deviation 7.93 17.40 77.49 12.56 19.71 88.56
CV (%) 8.37 8.11 10.01 12.79 10.09 10.55
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance