Rat DBP (Vitamin D Binding Protein) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0001942
🚚 Free UK Delivery on orders over £150
Processing The item has been added
Enquire For Bulk Order
✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short DescriptionThis rat DBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityRat
Sensitivity1.88ng/mL
Detection Limit3.13~200ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8Β°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolDbp
Gene ID24309
Uniprot IDDBP_RAT
SpecificityThis kit recognizes Rat DBP in samples. No significant cross-reactivity or interference between Rat DBP and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Tissue Specificity Expressed in the suprachiasmatic nuclei (SCN) and in most peripheral tissues, with a strong circadian rhythmicity.
Function This transcriptional activator recognizes and binds to the sequence 5'-RTTAYGTAAY-3' found in the promoter of genes such as albumin, CYP2A4 and CYP2A5. It is not essential for circadian rhythm generation, but modulates important clock output genes. May be a direct target for regulation by the circadian pacemaker component clock. May affect circadian period and sleep regulation.
Protein Name D Site-Binding Protein
Albumin D Box-Binding Protein
Albumin D-Element-Binding Protein
D Site Albumin Promoter-Binding Protein 1
Database Links
Cellular Localisation Nucleus
Alternative CLIA Names D Site-Binding Protein CLIA kit
Albumin D Box-Binding Protein CLIA kit
Albumin D-Element-Binding Protein CLIA kit
D Site Albumin Promoter-Binding Protein 1 CLIA kit
Dbp CLIA kit
Specificity This kit recognizes Rat DBP in samples. No significant cross-reactivity or interference between Rat DBP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells Γ—12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100Γ—) 96T: 1 vial, 120 ΞΌL 60 ΞΌL -20℃, 6 months
Concentrated HRP Conjugate (100Γ—) 96T: 1 vial, 120 ΞΌL 60 ΞΌL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8Β°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8Β°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8Β°C, 6 months
Concentrated Wash Buffer (25Γ—) 1 vial, 30 mL 2-8Β°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 97
EDTA plasma(n=8) 96-111 101
Cell culture media(n=8) 98-111 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 10.78 24.97 98.82 11.71 24.43 103.12
Standard deviation 1.12 2.84 9.63 1.27 2.74 11.31
CV (%) 10.39 11.37 9.74 10.85 11.22 10.97

Citations

Product Review

Well-cited Academic Publications
KO-Validated 700+ Lines
Technical Support Expert Scientific Team
1-Year Guarantee Antibodies & Proteins